QUANTITATIVE DETABWLINATION OF α- AMANITIN BY HPLC IN AMANlTA PHALLOIDES GROWING IN ISTANBUL FORESTS

In Turkey, mushroom poisonings are frequent especially in autumn and spring. In recent years, a great number of mushroom poisoning cases have been reported in fstanbul. Excursions to forests in fstanbul and its surroundings evidenced the widespread growth of Amanita phalloides in large numbers. The amount of α-amanitin was determined by HPLC in samples collected in 1991 and 1992.The a-amanitin content is between 4.4-13.3 mg/100 g in fresh mushrooms. Humidity and temperature are the most important factors affecting the amatoxin content in mushrooms.Key words: Amanita phalloides, α-amanitin, Mushroom poisoning, HPLC.
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QUANTITATIVE DETABWLINATION OF a - AMANHYN BY BPLC IN AMANlTA PH'OIIPES GROWING IN ISTANBUL FORESTS

In Turkey, mushroom poisonings are frequent especially in mtumn and spring. ,In recent years, a great number of mushroom poisoning cases have been reported in fstanbul. Excursions to forests in fstanbul and its surroundings evidenced the widespread growth of Amanita phalloides in large numbers. The amount of a-amanitin was determined by HPLC in samples collected in 1991 and 1992.The a-amanitin content is between 4.4-13.3 mg/100 g in fresh mushrooms. Humidity and temperature are the most important factors affecting the amatoxin content in mushrooms

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  • 1. Baytop, T., Tiirkiye'de Zehirli Bitkiler, Bitki Zehirlenmeleri ve Tedavi Yontemleri, p. 61, i.u.~ay. NO. 3560, istanbul(1989).
  • 2. Bresinsky, A., Besl,H., A Colour Atlas of Poisonous Fungi, p..,19, Wolfe Publishing Ltd. London (1990).
  • 3. Wieland, T., Peptides of Poisonous Amanita Mushrooms, p.13, Springer-Verlag, New York (1986).
  • 4. Tyler, V. E. Jr., Benedict, R. G., Brady, L. R., Robbers, J. E., J. Pharmacol. Sci. 55: 590- 593 (1966).
  • 5. Faulstich,H., Georgopoulos,D., Bloching, M., Wieland, T., Z. Naturforsch. 29: 86-88 (1974).
  • 6. Yocum R.R., Simons, D.M., Lloydia 40: 178-190 (1977).
  • 7. Andary,C., Enjalbert, F., Privat,G., Mandrou,B., J.Cliromatogr. 132: 525-532 0977).
  • 8. Andary, C., Enjalbert, F., Privat, G., Mandrou, B., Bull. Soc. Myc. Fr. 95: 169-180 (1979).
  • 9. Andary, C., Privat, G., Enjalbert, F., Mandrou, B., Documents Mycologiques 10: 61-69 (1979).
  • 10. Stijve, T., Seeger,R., Z. Naturforsch. 34: 1133-1138 (1979)
  • 11. Beutler, J.A., Der Marderosian, A.H., J.Nat.Prod. 44: 422-431 (1981).
  • 12. Miiderrisoglu, C., Karakullukqu, F., Orak, E., Beydilli, A., Mat, A., Tik TIP Demea Dergisi 58: 1-1 1 (1992).
  • 13. Yafet-Aji, D., Cahgkan, S., Nayu, A., Mat, A., Can, B., Yagar, Z., Ozgahin, H., Cullu, F., Sever, L., 1st. (;oc~ck Kli~z. Derg. 27: 51-54 (1992).