Antioxidants prevent indomethacin-induced oxidative damage in tongue tissues of rats

Background and Aims: Indomethacin (Indo) is an important nonsteroidal anti-inflammatory drug that has beneficial effects as a pain killer, but also has side effects. In this study, the protective effects of vitamins, selenium and ranitidine (Ran) against the side effects of Indo on tongue tissue were investigated. Methods: Rats were divided into six groups: the control group as group I; the group given a vitamin-selenium (Vitamin C, 100 mg/kg; Vitamin E, 100 mg/kg, beta carotene, 15 mg/kg, selenium, 0.2 mg/kg) combination as group II; the group treated with Indo as group III; the group administered vitamins-selenium and Indo (25 mg/kg, single dose) as group IV; the group given Ran (150 mg/kg) as group V; and the group given Ran and Indo as group VI. The experiments were done for 3 days. Animals received vitamins-selenium and Ran for 3 days, 2 h before the Indo administration (in same dose and time). Tongue tissues were taken and homogenized. Results: In the supernatants, glutathione levels, superoxide dismutase, catalase and glutathione-dependent enzyme activi- ties were reduced. Protein carbonyl, lipid peroxidation levels, lactate dehydrogenase and myeloperoxidase activities were increased in the group that was given Indo. Administration of vitamins-selenium and Ran reversed the levels and activities of the tested parameter in the Indo group. Conclusion: We suggest that vitamins, selenium and Ran have powerful protective effects on Indo-induced tongue injury in rats.

Keywords:

Beta carotene, Vitamin C, Vitamin E, Indomethacin Lingual Tissue, Selenium,

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