Valentina MURSALEYİVA, Akjunus IMANBAYEVA

Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides–Natural Source of Saponins

Allochrusa gypsophiloidesor Turkestansoap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitromass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium(MS)supplementedwithdifferentconcentrationsof6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS withkinetinledtoathree-foldreductioninthenumberofshoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration2.0mg/Linducedmaximalrooting(58 %and60%, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number

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