Nazim MAMEDOV, Wudeneh LETCHAMO, Thomas HARTMAN, André GOSSLİN, Lyle CRAKER

The Accumulation of Phenolic Compounds in Genetically Selected Amaranthus hybridus is Influenced by Endophytic Natural Growth Regulator

Amaranth (Amaranthus spp. L.) (Amaranthaceae), an endemic plant in Central and South America, grows worldwide, being cultivated in many temperate and tropical countries. Although several species of amaranth are frequently considered weeds, the plant is recognized as a food, constructive medicine, a source of protein and minerals livestock feed. The plant is widely cultivated, promoted, and increasingly consumed as a leafy vegetable and traditional medicine in Africa. Despite progressive genetic improvement and modern plant growing technologies, unfavorable climatic and ecological factors reduce the yield, and quality of the active plant botanicals. The role of bio-transformed endophytic microbial plant growth regulator formulation (BESF) on yield and accumulation of phenolic compounds in amaranth leaves is poorly understood. The current study assessed the effects of pre-sowing seed treatments with 0.0 %, 0.2 % and 0.4 % concentrations of BESF solution on germination, leaf yield, flavor and phenolic content in genetically selected Amaranthus hybridus var. cruentus. Data collected were subjected to analysis of variance (ANOVA). Significant treatment means were separated using Tukey test at p<0.05. BESF significantly increased fresh marketable leaf yield by over 360 kg/ha (29 %) compared to the control. The total flavonoid content in the leaves was raised by 34 % and 47 % with 0.2 % and 0.4 % BESF solution treatments respectively, compared to control. Maximum concentration levels of rutin, apigenin, apigetrin, and quercetin was obtained with 0.4% BESF solution treatment. An analysis of the collected data suggest that BESF was effective in overall improvement in leaf yield, chemical content, and flavor of A. hybridus var. cruentus, allowing us to recommend BESF application to raise A. hybridus var. cruentus leaves for nutrition and pharmacological applications.

Keywords:

apigenin growth regulator, nutraceutical, quercetin, rutin, symbiont,

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National Research Council (1984). Amaranth: Modern Prospects for an Ancient Crop. National Academy Press, Washington, D.C.
Jansen, V.R, Venter, W.S., Netshiluvhi, S.L., Van, D.H., & De Ronde, J.A. (2004). Role of indigenous leafy vegetables in combating hunger and malnutrition. South African Journal of Botany, 70(1), 52-59.
Grubben, G.J.H. (1976). The cultivation of amaranth as a tropical leaf vegetable with special reference to South Benin. Communication, Royal Tropical Institute, Amsterdam, 67, 207.
Lucas, B.O. (1988). The potential of leafy vegetables in Nigeria. Outlook on Agriculture 17 (4), 163-168.
Myers, R.L. (1996). Amaranth: New crop opportunity. Progress in New Crops. (J. Janick ed.), ASHS Press, Alexandria VA, 207-220.
Tindall, H.D. (1983). Vegetables in the Tropics. Macmillan Education Ltd. London 36-48.
Siegenthaler, I.E. (1960). Useful plants of Ethiopia. Imperial Ethiopian Collage of Agriculture and Mechanical Arts Jima Experiment Station Bulletin, 1(14), 14.
Ayalew, N.B. (2013). Growth and yield of Amaranthus hybridus L. subsp. cruentus (L.) Thell. grown on fields treated with different levels of urea and compost, M.S. Thesis at Addis Ababa University, Ethiopia.
Onyango M.C, Harbinson J, Imungi J.K, Shibairo S.I and van Kooten O. (2012). Influence of organic and mineral fertilization on germination, leaf nitrogen, nitrate accumulation and yield of vegetable amaranth (A. hypochondriacus). Journal of Plant Nutrition, 35(3), 342-365.
Anonym. (2017). Gardening in South Africa.
Mary, H.W. (2009). Too much of a good thing? Nitrate from nitrogen fertilizers and cancer. Rev. of Environ Health, 24(4), 357–363.
Hakeem, K.R, Sabir, M., Ozturk, M., Akhtar, M.S, Ibrahim, F.H, Ashraf, M, Ahmad, M.S.A. (2017). Nitrate and nitrogen oxides: sources, health effects and their remediation. Rev Environ Contam Toxicol., 242,183-217.
Petrini, O. (1991). Fungal endophytes of tree leaves. Microbial ecology of leaves, 179-197.
Aly, A., Debbab, A, Proksch, P. (2011). Fungal endophytes – secret producers of bioactive plant metabolites. Pharmazie, 68(7), 499-505.
Rodriguez, R., & Redman, R. (2008). More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. Journal of Experimental Botany, 59(5), 1109-1114.
Sieber, T. N. (2007). Endophytic fungi in forest trees: are they mutualists? Fungal Biology Reviews, 21(2-3), 75–89.
Letchamo, W., & Gosselin, A. (1996). Light, temperature, and duration of storage govern the germination and emergence of Taraxacum officinale seed. Journal of Horticultural Science, 71(3), 373-377.
Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
Zhishen, J., Mengcheng, T., Jianming, W. (1999). The determination of ﬂavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64, 555–559.
Mabry, T.J., Markham, K.R., and Thomas, M.B. (1970). The systematic identification of flavonoids, N.Y. Acad. Press.
Tomoko, K, Yoshiki, K., & Hideki, I. (2009). Rutin supplementation in the diet has protective effects against toxicant-induced hippocampal injury by suppression of microglial activation and pro-inflammatory cytokines. Cellular and Molecular Neurobiology, 29(4), 523–531.
Gins, M.S., Kolesnikov, M.P., Kononkov, P.F., & Gins, V.K. (2010). Characteristics of the accumulation of phenolic compounds in amaranth leaves under the effect of growth stimulators. Russian Agricultural Sciences, 36 (5), 349–352.
Wilson, D. (1995). Endophyte: the evolution of a term, and clariﬁcation of its use and deﬁnition. Oikos, 73, 274–276.
Döring, M. (2103). Overview about the research of endophytes as biocontrol agents against phytopathogens. Plant Protection and Plant Health in Europe 26-29 May. Berlin-Dahlem, Germany, 181.
Xiaolin, S., Hao. W. Zhenhao, Y., Meilan, L., & Chengri, Y. (2017). Endophytic bacteria isolated from Panax ginseng improves ginsenoside accumulation in adventitious ginseng root culture. Molecules, 22(837), 1-12.