Living-plants have been proven to have a potential for renewable energy source by embedding pairs of electrodes into it to allow flow of ions and hence generate electricity. Multiple tests using different type of electrodes and plants suggested that voltages are produced to greater or lesser extents where combination of copper (Cu)-zinc (Zn) and Aloe Vera produces the highest voltage output. To optimize the power output from the plant, a comprehensive knowledge regarding the mechanisms of energy generation is necessary. Initial hypothesis inferred this from electrochemistry process. Therefore, the presence of trace metals from the electrodes using Flame Atomic Absorption Spectroscopy (FAAS) was investigated in the plant to gain insight into the origin of the energy production. To further justify the stated hypothesis, comparison of trace metals concentration in electrodes immersed Aloe Vera between opened and closed circuit is also investigated. The obtained result confirmed that the electrochemistry process is responsible for the mechanism of the energy production from living plant.