Nutrient Composition, Anti-Nutritional Factors, and In Vitro Gas Production Assessment of Some Selected Legume Crops Stover Hay

Crop residues is cheapest way of making feed available to ruminant animals during dry season when there is non-availability of pastures. Consequently, a study was conducted to evaluate the nutritive value, anti-nutritional factors and in vitro gas production evaluation of five different legumes stovers (Bambaranut stover (T1), Groundnut stover (T2), Cowpea stover (T3), Soybean stover (T4) and Pigeon pea stover (T5)). The stovers were harvested at the University Teaching and Research Farms of Federal University Wukari between October and November. The harvested legumes stovers were chopped into 3–5cm length and air-dried under shed for two weeks on concrete floor. The chemical composition, phytochemical and in vitro gas production assessment were determined using standard procedure. The dry matter (DM) ranged from 91.83- 93.55%, crude protein (CP) 11.42-22.46%, Ash content (4.00 - 14.14%), crude fibre (CF) 19.30-31.05% while fibre fractions: Neutral detergent fibre (NDF) ranged from 52.50 – 59.80% and acid detergent fibre (ADF) 27.60–33.55%. All the parameters showed significant (P<0.05) differences across the treatments. The anti-nutritional factors such as Phenol (0.68-1.13%), Phytate (0.03- 0.06%), Oxalate (0.013- 0.020%), Saponins (0.09-0.18%), Trypsin (0.31-0.35%) and Tannin (0.56–0.82) varied significantly (P<0.05) except the saponins that was similar across the treatments. The results of in vitro gas production assessment indicate that all the parameter such as methane (CH4ml) 6-18ml, gas volume production (GVml) 9-28ml, metabolisable energy (ME MKJ/DM) 4.76-6.78, short chain fatty acids (SCFA µml) 0.16-0.61µml, organic matter digestibility (OMD %) 35.60 – 51.98%, dry matter degradability (DMD%) 57.65-86.63%, and fermentation efficiency (FE) 2.67-6.21 varied significantly(P<0.05) across the treatments.The results obtained from this study indicate that crude protein content of all the selected legumes stover hay is above 10-12% recommended for ruminant animals and anti-nutrition composition is within tolerance level. Furthermore, in vitro gas production assessment revealed that DMD, ME and SCFA of legumes stover hay is degradable and can supply energy to the ruminants. Thus, ruminant animals can utilise them if well processed and preserved for dry season feeding

Keywords:

Legumes stover hay, chemical composition anti-nutritional factors, in vitro gas evaluation,

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A.O.A.C. 2005. Association of Official Analytical Chemists. The official methods of Analysis 18th Edition. AOAC mc, Arlington. Virginia 1094pp.
A.R.C 1985. Agricultural Research Council. The Nutrient Requirements of Farm Animals. No. 2, Ruminants: Technology Review and Summaries. ARC, London.
Aganga, A.A., U.J. Omphile and T.F. Ntshontsi, 2005. Forage value of browses and its implication to traditional management of goats in Kgatleng district of Botswana. Journal Biology Science. 5: 506-510.
Ajayi, F.T., Odejide, J.O., Adesina, M. A.and Oluokun, J. A. 2011. Effects of time on the chemical composition of crop residues ensiled with urea. Proceedings 36th Conference, Nigeria Society for Animal, 13-16 March,2011,University of Abuja,Nigeria.
Ajayi, F.T., R.S. Akande, A.A. Adegbite and Idowu, B. 2009. Assessment of seven under-utilized grain legume foliages as feed resources for ruminants. Livestock Resource Rural Development. 21: 1-8.
Anele, U. Y. Arigbede, O. M. Sudekum, K. H. Ike, K. A. Oni, A. O. Olanite, J. A. Amole, G. A. Dele, P. A. ; Jolaosho, A. O., 2010. Effects of processed cowpea (Vigna unguiculata L. Walp) haulms as a feed supplement on voluntary intake, utilization and blood profile of West African dwarf sheep fed a basal diet of Pennisetum purpureum in the dry season. Animal Feed Science. Technology. 159 (1-2):10-17
Babayemi, O. J., Demeyer, D. and Fievez, V. 2004a. Nutritive value and qualitative assessment of secondary compounds in seeds of eight tropical browse, shrub and pulse legumes. Comm. Appl. Biol. Sci. 69 (1): 103-110.
Babayemi, O. J., Ekokotu, O. A and Iyang, A. U. 2009. Evaluation of ensiled cassava peels togeher with Albiza saman pods. Umoh,B.I, Udedibe, A.B.I, Solomon,I.R. Obasi,O.L., Okon, B.I. and Udoh.E.J., (Eds). Proceedings of the 34th Nigerian Society for Animal Conference, pp. 547-550.
Babayemi, O.J. and Igbekoyi J.A., 2010. In Eric Tielkes (Eds.), Competition for a resource in a changing world: New drive for rural development. Conference of the International Research on Food Security, Natural resources management and rural development, tropentag, 7-9th October 2010.
Barry, T.N. 1987. Secondary compounds of forages. In: Nutrition of herbivores. Hacker, J.B. and Ternouth, J.H. (eds.) A.P. Sydney pp. 91-120.
Beuvink and Spoelstral. 1992. Interactions between substrate, fermentation and products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro. Appl. Microbial Biotechnology 37, 507-509.
Binuomote, R. T and Babayemi, O. J. 2012. Nutritive evaluation of ensiled cassava (Manihot esculentus, Crantz) tops and guinea grass mixtures. Continental J. Animal and Veterinary Research 4 (2): Czerkawski, J. W. 1969. Methane Production in the Rumen and its Significance. World Rev. Nutr. Diet. 11. 240 – 282.
Blummel, M. and Becker, K. 1997. The degradability characteristics of fifty four roughages and roughage neutral detergent fibre as described in vitro gas production and their relationship to voluntary feed intake. Brit. J. Nutr. 77: 757-768.
Blummel, M. and Ørskov, E. R. 1993. Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Anim. Feed Sci. Technol. 40:109 119.
Bohn L, Meyer, A.S. and Rasmussen S.K. 2008."Phytate: impact on environment and human nutrition. A challenge for molecular breeding. Journal of Zhejiang University. Science. B.9 (3): 16591.
Cammack, Richard. 2006. Oxford dictionary of biochemistry and molecular biology. Oxford: (Revised edition.). Oxford University Press. 2006. p. 47.
Cheeke, P.R. and Shull, L.R. 1985. Natural Toxicants in Feeds and Livestock. AVI Publishing Inc., West Port, Connecticut.
Czerkawski, J. W. 1969. Methane Production in the Rumen and its Significance. World Rev. Nutr. Diet. 11. 240 – 282.
Das, T.K., Banerjee, D., Chakraborty. D., Pakhira, M.C., Shrivastava, B. & Kuhad, R.C., 2012. Saponin: Role in animal system. Vet. World 5, 248-254.
Diagayette, M. and W. Huss. 1981. Tannin contents of African pasture plants. Effects on analytical data and in vitro digestibility Animal Research Development. 15:79-90.
Duncan, D. B. 1955. Multiple range and multiple F-test. Biometrics.11:1- 42
Ekholm P, Virkki L, Ylinen M, Johansson, L. 2003. "The effect of phytic acid and some natural chelating agents on the solubility of mineral elements in oat bran". Food Chemistry.80 (2).
Falola, O. O., Alasa, M. C., Amuda, A. J., Babayemi, O. J. 2013. Nutritional and Antinutritional components of vetiver grass (Chrysopogon zizanioides L. Roberty) at different stages of growth. Pakistan Journal of Nutrition 12 (11): pp 957-959
Farquer, J. N. 1996. Plant sterols. Their biological effects in humans. Handbook of lipids in human nutrition. BOCA Raton FL CRC Press, pp. 101-105.
Fellner, V. 2004. In vitro versus in vivo estimates of ruminal fermentation. Department of animal science, North Carolina state university.
Fievez, V., Babayemi, O. J. and Demeyer, D. 2005. Estimation of direct and indirect gas production in syringes: a tool to estimate short chain fatty acid production requiring minimal laboratory facilities. Animal Feed Science Technology. 128 – 124, 197 – 210.
Francis, G., Makkar, H.P.S., & Becker, K. 2001. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199: 197-227.
Gatenby, R. M. (2002). Sheep Revised Edition. Tropical Agricultural Series. Macmillan Publishers Ltd. Malaysia. Pp.1 – 178.
Gebremariam, T. and Belay, S. 2016. Livestock feed resources utilization practices in Tanqua-Abergelle district of Tigray, northern Ethiopia. Tropical Animal Health and Production, 48(6):1183-1190.
Getachew, G., Blummel, M., Marker, H. P. S. and Becker, K. 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds. A review. Animal Feed Science Technology, 72: 261 – 281
Getachew, G., Marker, H. P. S. and Becker, K. 1999. Stoichiometric relationship between short chain fatty acid and in vitro gas production in presence and absence of polyethylene glycol for tannin containing browses, EAAP Satellite Symposium, Gas production, fermentation kinetics for feed evaluation and to assess microbial activity, 18 – 19 August, Wageningen, The Netherlands.
Gillespie, J. R. 1998. Animal Science Delmar Publishers. International Thonysson Publishing Company, p. 1204.
Goel, G. & Makkar, H. P. S., 2012. Methane mitigation from ruminants using tannins and saponins. Trop. Anim. Health Prod. 44, 729-739.
Guillaume, J.C., Kaushik, S., Bergot, P. & Métailler, R. 1999. Nutrition et alimentation des poissons et des crustacés. Inra, Paris – Ifremer, Issyles Moulineaux (co Eds), 489 pp.
Hess, H. D., Kreuzer, M., Diaz, T. E., Lascano, C. E., Carnulla, J. E., Soliva C. R. and Machmullar, A. 2003. Saponin rich tropical fruits affect fermentation and methanogenesis in faunated and defaunated rumen fluid. Anim. Feed Sci. Technol., 109: 79-84.
Holter, .J. B. and Young, A. J. 1992. Methane production in dry and lactating Holstein Cows. J. Dairy Science. 75: 2165 – 2175.
Jingura, R.M., Sibanda, S. & Hamudikuwanda, H. 2001. Yield and nutritive value of tropical forage legumes grown in semi-arid parts of Zimbabwe. Tropical Grass. 35, 168-174.
Kellems, R. O. and Church, D. C. 1998. Livestock Feeds and Feeding (4th edition). Prentice-Hall Inc., New Jersey, USA.
Lanyasunya, T.P., W.H. Rong, S.A. Abdulrazak, P.K. Kaburu, J.O. Makori, T.A. Onyango and D.M. Mwangi. 2006. Factors limiting use of poultry manure as protein supplement for dairy cattle on smallholder farms in Kenya. International Journal Poultry Science, 5: 75-80.
Maga, J.A. 1983. Phytate; its chemistry, occurrence, food interactions nutritional significant and method analyses, Journal of Agricultural Food Chemistry, 30: 1-9.
Mahala, A.G., Amasiab, S.O., Yousif, M.A. & Elsadig, A. 2012. Effect of plant age on DM yield and nutritive value of some leguminous plants (Cyamopsis tetragonoloba, Lablab purpureus and Clitoria (Clitoria ternatea)). Int. Res. J. Agri. Sci. Soil Sci. 2, 502-508..
McDonald, P. Edwards , R.A. , Greenhalgh, J. F. D., Morgan, C.A., Sinclair, L. A. and Wilkinson, R. G. 2010. Animal Nutrition 7thEdn. Pp 507.
McDonald, P., Edward, R.A. Greenhalgh, J. F. D. and Morgan, C. A. 1995. Animal Nutrition. 5th Ed. Longman Scientific and Technical, England.
Meale, S.J., Mcallister, T.A., Beauchemin, K.A., Harstard, O.M. & Chaves, A.V., 2012. Strategies to reduce greenhouse gases from ruminant livestock. Acta Agriculturae Scandinavica Anim. Sci. 62, 199-211.
Menke, K. H. and Steingass, H. 1988. Estimation of the energetic feed value from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Devt. 28: 7 - 55.
Menke, K. H., Rabb, L., Salewski, A., Steingass, H., Frit, F. and Schneider, W. 1979. The estimation of the digestibility and metabolisable energy content of ruminants feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci., 93: 217-222
Moalafi, A.I., J.A.N. Asiwe and S.M. Funnah, 2010. Germplasm evaluation and enhancement for the development of cowpea (Vigna unguiculata (L.) Walp dual-purpose F2 genotypes. Afr. J. Agric. Res., 5: 573-579.
Molyneux, R.J., Stevens, K.L. and James, L.F. 1980. Chemistry of toxic range plants. Volatile constituents of Broomweed (Gutierrezia sarothrae). Journal of Agriculture and Food Chemistry 28: 1332-1333.
Motubatse, M.R., J.W. Ngambi, D. Norris and M.M. Malatje, 2008. Effect of polyethylene glycol 4000 supplementation on the performance of indigenous Pedi goats fed different levels of Acacia nilotica leafmeal and ad libitum Buffalo grass hay. Trop. Anim. Health Prod., 40: 229-238.
Mueller-Harvey, I., 2005. Tannins in animal health and nutrition opportunities for temperate and tropical regions. In: M.R.P. Wanapat (ed.). Proceedings of International Symposium on Integrating Livestock-Crop Systems to Meet the Challenges of Globalisation. Khon Kaen, Thailand. pp. 448-450.
Mupangwa, J.F., 2000. Nutritive value, intake and utilisation of forage legumes in ruminants. Ph.D. thesis. University of Zimbabwe.
Natis, A. S. and J. C. Malachek, 1981. Digestion and utilization of nutrients oak browse by goats. Journal of Animal Science. 52: 283-288.
Njidda, A. A., Ikhimioya, I. and Babayemi, O. J. 2010. Variation of 24hr In vitro gas production and estimated metabolisable energy values of ruminants feeds. Babayemi, O.J., Abu, O.A. and Ewuola, E.O. (Eds): Proceedings of the 35th Nigerian society for animal production 14th – 17th March 2010, pp. 597 – 600.
NRC. 1985. Nutrients Requirements of beef cattle (17th edition) National Academy Press, Washington D.C. USA.
NRC. 2001. National Research Council. Nutrient Requirements of Sheep.7th Revised Edition. National Academy Press, Washington DC.
Nwokolo, E. N. and B. B. Bragg, 1977. Influence of phytic acid and crude fibre on the availability of minerals from protein supplements in growing chicks. Journal of Animal Science, 57:475-477.
Ofokansi, K. C., Esimone, C. O. and Anele, C. K. 2005. Evaluation of the in vitro combined antibacterial effects of the leaf extracts of Bryophyllum pinnatum (Farm Crassulaceae) and Ocimum gratissimum (Farm Labiate). Plant Prod. Res. J. 9: 23-27.
Oke, O.L. 1969. Oxalic acids in plants and in nutrition. World Rev. Nutr. Dietetics, 10: 362-303.
Okwu, D. E. and Omodamiro, O. D. 2005. Effects of hexane extract and phytochemical content of Xylopia aethiopia and Ocimum gratissimum on the uterus of guinea pig. Biol. Res. Volume 3 (2):40-44.
Ørskov, E. R. and Ryle, M. 1990. Energy Nutrition in Ruminants. Elsevier, London. Pp. 149.
Ørskov, E. R. and McDonald, L. 1979. The estimation of Protein degradation ability in the rumen from incubation measurement weighed according to rate of passage. Journal of Agricultural Science. Cambridge. 92:499 – 503
Oyatayo T. K., Songu A. G., Amos A. G. and Ndabula C. 2015. Assessment of heavy metal concentration in hand dug well water from selected land uses in Wukari Town, Wukari, Taraba state Nigeria. Journal of Geoscience and Environmental protection (3) 1-10.
Rinne, M. Jaakkola, S., and Huhtanem, P. 1997. Grass maturity effects on cattle fed silage-based diets.1 Organic matter digestion, rumen fermentation and nitrogen utilisation. Animal Feed Science Technology. 67: 1– 17.
Roger Y. Stainier, Edward, A. Adelberg and John L., Ingraham. 1977. General Microbiology. Published by The Macmillan Press Ltd.
Rosenthal, G. A. and Janzen, D. H. 1979. Herbivores: Their interaction with secondary plant metabolites Academic press Pg. 4, 25.
Rusdy, M. 2016. Elephant grass as forage for ruminant animals. Livestock Research for Rural Technical 28(8) http://www.Irrd28/4/rusd28049.ht ml.
Saadullah, M. Haque, M. and Dolberg, F. 1981. Effectiveness of ammonification through urea in improving the feeding value of rice straw in ruminants. Tropical Animal Production. 6: 3036.
Singh B B, Musa A, Ajeigbe H A and Tarawali, S. A. 2011. Effect of feeding crop residues of different cereals and legumes on weight gain of Yankasa rams. International Journal of Livestock Production, 2(2):017-023.
Singh, G. P. and Oosting, S. J. 1992. A net carbohydrate and protein system for evaluating cattle diets. I. Ruminating fermentation, Journal of Animal Science. 70:35551 – 3561.
Sofowora, A. O. 1993. Phyto-Chemical Screening of Medicinal Plants and Traditional Medicine in Africa. Edition. Spectrum Book Ltd., Nigeria, 150 – 156.
Somart, K., Parker, D. S., Poulison, P. and Wanapat, M. 2000. Fermentation characteristic and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asia – Aust. Journal of Animal Science. 13:1084 –1094
Tacon, A. G. J. 1997. Fish meal replacers: review of antinutrients within oilseeds and pulses - a limiting factor for the aquafeed green revolution? In: Tacon A., Basurco B., (Eds.), pp. 153-182. Feeding tomorrow’s fish. Vol. 22. Cahiers Options Méditerranéennes, Institut Agronomique Méditerranéen de Zaragoza, Spain.
Tesfaye Z. G, Anal, A K and Gebreyohanis, G. 2012. Assessment of the sheep production system of northern Ethiopia in relation to sustainable productivity and sheep meat quality. International Journal of Advanced Biological Research, 2(2):302-313.
Tolera, A, Merkel, R. C., Goetsch, A. L., Sahlu T and Negesse, T. 2000. Nutritional constraints and future prospects for goat production in east Africa. pp. 43-57. In: Merkel R C, Abebe G and Goetsch A L (eds.), The opportunities and challenges of enhancing goat production in east Africa. Proceedings of a conference held at Debub University, Awassa, Ethiopia from November 10 to 12, 2000.E (Kika) de la Garza Institute for Goat Research, Langston University, Langston.
Van Soest P. J. 1994. Nutritional Ecology of the Ruminant; Comstock Publishing Associates A division of Comell University press. LTTIECA and London.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber-neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74, 3583–3597
Wanapat, M., Cherdthong, A., Phesatcha, K. & Kang, S. 2015. Dietary sources and their effects on animal production and environmental sustainability. Anim. Nutri. 1, 96-103.