Kinetics and thermodynamics of lipids extraction from microalgae using n-hexane

The growth kinetics and bioenergetics of microalgae is well studied; however, the kinetics and thermodynamics of extraction of lipids from microalgae is poorly understood. The present study focuses on the kinetics and thermodynamics of the lipid extraction process from microalgae Chlamydomonas reinhardtii using n-hexanein a Soxhlet extractor. The extraction process was shown to increase lipids extraction with temperature (from 35 to 55 °C). Further, at a given temperature, the percent lipid extraction is linearly increased till 1.5 hours and then remained almost constant. The statistical analysis including parameters like correlation coefficient (R2), the root mean square (RMS), standard deviation (SD) and standard error of estimation (SEE) were used to establish the relevance of each model. The series of best kinetic models from high to low prominence is pseudo-second order, hyperbolic, Elovich’s, parabolic and power model. The enthalpy and entropy of the present system is 266.31 kJ/mol and 0.924 kJ/mol-K, respectively. The Gibb’s free energy decreased from -19.053 to -37.412 kJ/mol as the extraction temperature increased. The kinetic and thermodynamics parameters evaluation suggested that the microalgal lipid extraction using n-hexane is efficient and a spontaneous process.

Keywords:

Microalgae, Lipids, Extraction Solvent, Non-Linear Models,

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