Barley is a very important animal feed plant in terms of protein content as well as plant nutrients and vitamins it contains. In this study, it was aimed to determine and compare macro (N, P, K, Ca and Mg) and micro-nutrients (Fe, Mn, Zn and Cu), and protein contents of local barley varieties of Black Sea Region. For this purpose, leaf and grain samples were taken from 17 barley varieties. Total N, P, K, Ca, Mg, Fe, Mn, Zn and Cu were analysed in the leaves and grain samples, and crude protein contents were determined in the grain samples. It was determined that the total N, P, K, Ca, Mg, Fe, Mn, Zn and Cu contents of leaf samples of local barley varieties of Black Sea Region were 2.50- 4.10 %, 0.03- 0.06 %, 0.78-1.65 %, 0.95-1.87 %, 0.19-0.34 %, 47.4-246.7 mg/kg, 37.8-84.5 mg/kg, 7.7-14.0 mg/kg and 4.0-7.4 mg/kg, respectively. It was found that the protein, total N, P, K, Ca, Mg, Fe, Mn, Zn and Cu contents of the grain samples of the barley varieties were 10.2-16.0 %, 1.75-2.74 %; 505.1-837.5 mg/kg, 3822-5652 mg/kg, 651-46380 mg/kg, 1587-2413 mg/kg; 12.2-474.2 mg/kg; 11.2-23.6 mg/kg; 13.1-20.9 mg/kg and 2.3-4.0 mg/kg, respectively. As a result, it was determined that the leaf and grain samples of the local barley varieties in Black Sea Region of Turkey were determined to vary in terms of plant nutrient content, and that it can be used as animal feed because it was rich in protein content.