ALLELOPATHIC EFFECTS OF OLIVE OIL MILL WASTEWATER (OMW) ON SAINFOIN (Onobrychis viciifolia Scop.) GERMINATION

In this study, the olive mill wastewater at different concentrations (control, 1/1, 1/2, 1/3, 1/4 (OMW/distilled water) and undiluted OMW) was used to determine the impact to the sainfoin seed germination. In the study, the germination ratio and mean germination time showed statistically significant differences in terms of levels according to treatments, sainfoin populations and sainfoin populations x treatments interaction (p<0.01), germination ratio varied at 5-70 % and the highest value (70 %) was obtained from 1/2 (OMW/distilled water) application in true sainfoin seeds. In general, the OMW (added distilled water) treatments were increased germination ratio. The lowest germination ratio (11.67 %) was determined in sainfoin seeds collected from Eğirdir location. According to application, the highest germination ratio was obtained from 1/1 and 1/2 (OMW/distilled water) applications. In experiment, mean germination time varied in 5.0-11.0 days. Generally, high OMW concentrations (except undiluted OMW) extended mean germination time in sainfoin seeds. In all OMW applications, it shortened in ES seeds, but not in TS seeds. Additionally, germination time weren’t effected as positive from OMW applications (except undiluted OMW) in TS seeds. The longest mean germination time (9.0 day) was in ES seeds. According to treatments, germination in the shortest time occurred in 1/4 (OWM/distilled water) treatment. As a result, olive oil wastewater (OMW) mixed to irrigation water in certain dozes may increase seed germination and can be used as fertilizer in agriculture.

Keywords:

Allelopathic germination, olive mill wastewater,

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Akgun I., Altindal N., Karadogan T., 2007. Effect of GA3 and KNO3 applications on seed germination in sainfoin (Onobrychis sativa Lam. and Onobrychis ssp). Turkish 7th. Field Crops Congress, Erzurum/Turkey. 25-27 June 2007, 230-234.
Altindal N., Altindal D., 2013. Allelopathic effect of sage and Turkish oregano volatile oils on in vitro in sainfoin (Onobrychis viciifolia). Journal of Essential Oil Bearing Plants, 16(3): 328-333.
Anonymous 2018a. Sainfoin haylage. Available at http://www.hydehallhaylage.co.uk/index.html.
Anonymous 2018b. Fact sheet-USDA plants database. Available at http//www. plants.usda.gov/factsheet/pdf/fs_onvi.pdf. Artola A., Cariilo-Castaneda G., Garcia de los Santos G., 2003. Hydropriming: A strategy to increase Lotus corniculatus L. sees vigor. Seed Sci. and Technol., 31:455-463.
Ashrafi E., Razmjoo K., 2010. Effects of priming on seed germination and field emergence of safflower (Carthamus tinctorius L.). Seed Sci. Technol., 38:675-681.
Aybeke M., Olgun G., Sıdal U., Kolankaya D., 2000. Zeytinyağı fabrikası atık suyunun buğday (Triticum aestivum L.) kök ucu hücrelerindeki mitoz bölünme ve total protein miktarı üzerine etkisi. Turkish Journal Biology, 24:127-140.
Basra S.M.A., Zia M.N., Metmood T., Afzal I., Khaliq A., 2002. Comparison of different invigoration techniques in wheat (Triticum aestivum L.) seeds. Pakistan Journal Arid Agric., 5:11-16.
Buchmann C., Felten A., Peikert B., Munoz K., Bandow N., Dag A., Schaumann G.E., 2015. Development of phytotoxicity and composition of a soil treated with olive mill wastewater (OMW): an incubation study. Plant Soil, 386:99-112.
Carleton A.E., Cooper C.S., Wiesner L.E., 1968. Effect of seed pod and temperature on speed of germination and seedling elongation of sainfoin (Onobrychis viciaefolia Scop.). Agronomy Journal, 60(1):81-84.
Cavallaro V., Maucineri C., Barbera A.C., 2014. Lolium multiflorum Lam. cvs germination under simulated olive mill wastewater salinity and pH stress. Ecological Engineering, 71:113-117.
Çetin M., 2009. Zeytin karasuyunun aerobik stabilizasyonunda biyo-ayrışma kinetiğinin belirlenmesi. Yüksek Lisans Tezi. Balıkesir Üniversitesi, Fen Bilimleri Enstitüsü, Çevre Mühendisliği Anabilim Dalı, 127 sayfa.
Das G., Sen-Mandi S., 1992. Scutellar amylase activity in naturally aged and accelerated aged wheat seeds. Ann. Bot., 69:497-501.
Ditterline R.L., 1973. Yield and yield components of sainfoin (Onobrychis viciaefolia Scop.) seed and an evaluation of its use as a protein supplement. Montana States University, Crop and Soil Science, Doctor Philosopy, Bozeman, Montana. 96 pp.
Frame J., 2007. Onobrychis viciifolia Scop. Available at http://www.fao.org/AG/agpc/doc/Gbase/DATA/Pf000347.html.
Gebreyohannes A.Y., Mazzei R., Giorno L., 2016. Trends and current practices of olive mill wastewater treatment: Application of integrated membrane process and its future perspective, Separation and Purification Technology, 163:45-60.
Gray F.A., 2006. Shoshone' sainfoin, plant sciences timely information series. Department of Plant Sciences, College of Agriculture, University of Wyonming, No:2, July 2006, Available at http://cordis.europa.eu/result/rcn/161034_en.html.
Helland M.H., Wicklund T., Narvhus J.A., 2002. Effect of germination time on alpha-amylase production and viscosity of maize porridge. Food Res. Int., 35:315-321.
Isidori M., Lavorgna M., Nardelli A., Parrella A., 2005. Model study on the effect of 15 phenolic olive mill wastewater constituents on seed germination and Vibrio fischeri metabolism. J. Agric. Food Chem., 53 (21):8414-8417.
Krogmeier M.G., Bremner J.M., 1989. Effects of phenolic acids on seed germination and seedling growth in soil. Biology and Fertility of Soils, 8, 116-122.
Mackay W.A., Davis T.D., Sankhla D., 1995. Influence of scarification and temperature treatments on seed germination of Lupinus havardii. Seed Science & Technology, 23:815-821.
Mseddi S., Chaari L., Belaid C., Chakchouk I., Kallel M., 2015. Volarization of treated olive mill wastewater in fertigation practice. Environ Sci Pollut Research, DOI 10.1007/s11356-015-4353-6. Muscolo A., Sidari M., Mallamaci C., Attina E., 2010. Effect of olive mill wastewater on seeed germination and seedling growth. Terr Aquat Environ Toxicol., 4 (1):75-83.
Naija D.S., Boussaadia O., Dhiab A.B., Mariem F.B., Braham M., 2014. Valorization of the olive sector effluents as potential fertilizers and their ımpact on biological, physical and chemical properties of the soil. Research Journal of Agriculture and Environmental Management, 3(9):450-459.
Nandi S., Das G., Sen-Mandi S., 1995. β-amylase activity as an index for germination potential in rice. Ann Bot., 75:463-467.
Noorbakhshian S.J., Nabipour M., Meskarbashee M., Amooaghaie R., 2011. Evaluation of germination and biochemical indexes of sainfoin hydroprimed seed. American-Eurasian J. Agric.&Environ. Sci., 11 (5):671-678.
Ouzounidou G., Asfi M., 2012. Determination of olive mill wastewater toxic effects on three mint species grown in hydroponic culture. Journal of Plant Nutrition, 35:726-738.
Peel M.D., Asay K.H., Johnson D.A., Waldron B.L., 2004. Forage production of sainfoin across an irrigation gradient. Crop Science, 44:614-619.
Pérez J.D., Esteban E., Gomez M., Gallardo L.F., 1986. Effects of waste water from olive processing on seed germination and early plant growth of different vegetable species. Journal of Environmental Science and Health, Part B 21, 349-357.
Rahmanian N., Jafari S.M., Galanakis C.M., 2014. Recovery and removal of phenolic compounds from olive mill wastewater. Journal of the American Oil Chemists' Society, 91:1-18.
Ruan S., Xue Q., Tylkowska K., 2002. The influence of priming on germination of rice (Oryzo sativa L.) seeds and seedling emergence and performance in flooded soil. Seed Science & Technology, 30:61-67.
Rusan M.J., Albalasmeh A.A., Zuraiqi S., Bashabsheh M., 2015. Evaluation of phytotoxicity effect olive mill wastewater treated by different technologies on seed germination of barley (Hordeum vulgare L.). Environ Sci Pollut Res., 22(12):9127-35.
Spedding C.R.W., Diekmahns E.C., 1972. Grasses and legumes in British agriculture. Bulletin No. 49, Commonwealth Bureau of Pastures and Field Crops, Farnham Royal, 511 pp.