Yüksek Miktarlarda Şeker Pancarı Melasının Hidrojen ve 5-Aminolevulinik Asit Üretimi için Substrat Olarak Değerlendirilmesi

Şeker pancarı melası değerli bir hammaddedir ve sükroz başta olmak üzere yüksek miktarda şeker içerir. Bu nedenle, mikroorganizmalar tarafından son derece değerli kimyasalların üretimi için substrat olarak kullanılabilir. Bu çalışmada, Rhodobacter sphaeroides O.U.001'in çoğalması ile hidrojen ve 5-aminolevulinik asit (5-ALA) üretimlerini arttırıp arttırmadıklarını araştırmak için ilk kez oldukça yüksek melas konsantrasyonları test edilmiştir. İlk önce, şeker içeriği 34 g/L, 41 g/L, 48 g/L, 55 g/L ve 61 g/L olan beş farklı ortam, melas kullanılarak hazırlandı. Daha sonra, kesikli süreçlerle, bu ortamlarda bakteri çoğalması ile hidrojen ve 5-ALA üretimleri incelenmiştir. Sonuç olarak, bugüne kadarki en yüksek hücre çoğalması (OD660: 9.26, 4.54 g cdw/L), 34 g/L şeker içeren ortamda elde edildi. Benzer şekilde, aynı çoğalma kültüründe bugüne kadarki en yüksek miktarda 5-ALA (37.44 mM) elde edildi. Bu önemli gelişmelere ek olarak, 34 g/L şeker içeren ortamdan maksimum 21.02 mL (0.42 L H2/L) hidrojen toplanmıştır. Sonuç olarak, 34 g/L'lik bir şeker konsantrasyonunun kullanılması, şimdiye kadarki en yüksek bakteri üremesini ve 5-ALA oluşumunu sağladı. Ayrıca, önemli miktarda hidrojen üretimini de destekledi.

Anahtar Kelimeler:

5-aminolevulinik asit, hidrojen, melas, Rhodobacter sphaeroides

Evaluation of High Concentrations of Sugar Beet Molasses as Substrate for Hydrogen and 5-Aminolevulinic Acid Productions

Sugar beet molasses is a valuable raw material and it contains high amount of sugar especially sucrose. Therefore, it could be used as substrate for the generation of highly valuable chemicals by microorganisms. Here, considerably high concentrations of molasses were tested for the first time to investigate if they could enhance the growth of Rhodobacter sphaeroides O.U.001 and generations of hydrogen and 5-aminolevulinic acid (5-ALA). Firstly, five distinct growth cultures having sugar contents of 34 g/L, 41 g/L, 48 g/L, 55 g/L and 61 g/L were made ready using molasses. Then, in batch processes, bacterial growth and generations of hydrogen and 5-ALA were investigated in these media. As a result, the highest cell growth (OD660: 9.26, 4.54 g cdw/L) to date was achieved in 34 g/L sugar containing medium. Similarly, the highest quantity of 5-ALA (37.44 mM) to date was attained in the same growth culture. In addition to these significant improvements, at maximum 21.02 mL (0.42 L H2/L) of hydrogen was collected from 34 g/L sugar containing medium. To conclude, using a sugar concentration of 34 g/L yielded the highest bacterial growth and 5-ALA generation so far. And, it also supported the generation of considerable amount of hydrogen.

Keywords:

5-aminolevulinic acid, hydrogen, molasses, Rhodobacter sphaeroides,

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