BİYOMÜHENDİSLİKTE KULLANILAN KAN ANALOGLARINA GENEL BİR BAKIŞ

Biyomühendislikte yapılan çalışmalarda in vitro deneyler için gerçek kanın kullanılması; elde edilmesi, saklanması, manipülasyonu, büyük miktarlarda gerekli olması, hava ile temas ettiğinde yapısının değişmesi ve toksisitesi gibi nedenlerden dolayı pek mümkün değildir. Bu yüzden in vitro ortamda yapılan deneylerde kan yerine kullanılacak sıvıların araştırılması önemli bir konudur. Bu sıvıların insan kanına benzer reolojik özellikler göstermesi beklenir. Fakat kan reolojisi son derece karmaşık olduğundan, kanın tüm reolojik özelliklerini karşılayan analog sıvılar geliştirmek oldukça zordur. Tek bir analog sıvısı ile kanın bütün özellikleri aynı anda sağlanamadığından, laboratuvar ortamında yapılacak çalışmanın özelliğine bağlı olarak kan yerine geçecek farklı kan analoglarının seçimi yapılmaktadır. Yapılan çoğu çalışmalarda, bu kan analogları için hazırlanan bileşimlere Xanthan Gum (XG) ilavesiyle kanın reolojik özelliklerine en yakın davranış sergileyen analoglar ön plana çıkmaktadır. Bu çalışmamızda in vitro koşullarda kanın yerine geçebilecek kan analog sıvılarının araştırılması yapılmış, bu analogların reolojik özellikleri tablolarla sunulmuş ve önerilerde bulunulmuştur.

Anahtar Kelimeler:

Kan Analoğu, Reoloji, Biyomühendislik, Xanthan Gum

An Overview of Blood Analogues Used in Bioengineering

The use of real blood for in vitro experiments in bioengineering studies is unlikely not possible due to reasons as; obtaining, storing, manipulating, being required in large quantities, changing of the structure when exposed to air and toxicity. Therefore, it is an important issue to investigate the fluids that will be used instead of blood in the in vitro experiments. These fluids are expected to exhibit rheological properties similar to human blood. However, as blood rheology is extremely complex, it is difficult to develop blood analogue fluids that meet all rheological properties of blood. Since all properties of blood cannot be achieved at the same time with a single analogue fluid, depending on the characteristics of the study in laboratory environment different blood analogues are selected to replace blood. In most studies, analogues exhibiting the closest behavior to the rheological properties of the blood come to the fore with the addition of Xanthan Gum (XG) to the compositions prepared for these blood analogs. In this study; blood analogue fluids that can replace blood in in vitro conditions have been investigated, the main characteristics of these analogs have been presented with tables and suggestions have been made.

Keywords:

Blood Analog, Rheology, Bioengineering, Xanthan Gum,

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