Songül Budak DİLER, Fikriye POLAT, Eyüp ÖZER

Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi

Kenodeoksikolik asitin 7b epimeri olan ursodeoksikolik asit (UDKA), kolestatik karaciğer hastalıklarının

Anahtar Kelimeler:

Genotoksik etki, İnsan periferal lenfositleri, Kromozom aberasyon testi, Mitotik indeks, Ursodeoksikolik asit

In Vitro Genotoxic Effects of Ursodeoxycholic Acid in Human Peripheral Blood Lymphocytes

Ursodeoxycholic acid (UDCA), the 7b epimer of chenodeoxycholic acid has been increasinglyused for the treatment of cholestatic liver diseases. In this study, it was aimed to determine the potential in vitrogenotoxic effects of ursodeoxycholic acid in human peripheral blood lymphocytes. The potential genotoxic andcytotoxic effects of UDCA were investigated in vitro by using chromosome aberration and mitotic index assays.Human peripheral blood lymphocytes were treated with 10, 50 and 100 μg/ml of UDCA for 24 and 48 hours. Thedata were evaluated using one-way ANOVA (Post Hoc Analysis-LSD Test) test in the SPSS statistics program.Statistical results showed that the applied doses of UDCA did not cause a significant increase in chromosomeaberration frequency, and also it did not cause a significant decrease in mitotic index when compared to the control(p>0.05). The finding that UDCA does not cause an increase in chromosomal aberrations may be related to theproduction of this substance physiologically as bile acid in the human body, even in trace amount. The fact thatthere are many patients using UDCA increases the importance of findings obtained from this study.

Keywords:

Chromosome aberration test, Genotoxic effect, Human peripheral lymphocytes, Mitotic index, Ursodeoxycholic acid,

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