Tc-99m ile İşaretli Kurkumin Yüklü Katı Lipit Nanopartiküllerin Karaciğer-Dalak Sintigrafsindeki Rolü
Son zamanlarda, hastalıkların tanı ve tavisini kolaylaştırmak amacıyla, nanoteknoloji vemoleküler görüntüleme alanları, çok fonksiyonlu nanopartiküllerin oluşturulması ile birleştirilmiştir.Nanopartiküllerden oluşturulan yeni nesil görüntüleme ajanlarının sahip olduğu çok yönlü ve eşsizözelliklerinden faydalanan alanlardan biriside nükleer tıp alanıdır. Yapılan bu çalışmada; kurkuminyüklü katı lipit nanopartiküllerin (K-KLN) karaciğer ve dalak sintigrafsinde kullanılabilirliliğiaraştırıldı. Öncelikle, kurkumin yüklü katı lipit nanopartiküller (K-KLN) mikroemülsiyon ve düşüksıcaklıkda katılaştırma yöntemiyle hazırlandı. Lazer kırınımı (LD) analizi ile partikül boyutu belirlendi.Termogravimetrik analizi (TGA) ile K-KLN’lerin sıcaklığa karşı stabil olduğu gözlemlendi. Daha sonraK-KLN’ler Teknesyum-99m (99mTc) ile radyoaktif olarak işaretlendi. İnce katman kromatograf yöntemiile radyoaktif işaretleme etkinliği %95’den daha fazla olarak bulundu. 99mTc-K-KLN’ler intravenöz (i.v.)olarak tavşana enjekte edildi ve gama kamera ile dinamik görüntüler kaydedildi. Elde edilen dinamikgörüntülerde karaciğer ve dalak belirgin bir şekilde görüntülendi. Sintigrafk bulgulara ek olarakyapılan ex vivo biyodağılım çalışmaları ve floresan mikroskop çalışmalarında karaciğer ve dalakta99mTc-K-KLN’lerin varlığı gözlemlendi.
The Role of Curcumin-Loaded Solid Lipid Nanoparticles Labeled with Tc-99m in the Liver-Spleen Scintigraphy
Recently, nanotechnology and molecular imaging felds are integrated to facilitate the diagnosis andthe treatment of diseases with create multi-functional nanoparticles. Nuclear medicine is one of the felds that beneftfrom the multi-faceted and unique characteristics of new generation imaging agents that are made of nanoparticles.This study investigated the feasibility of using curcumin-loaded SLNs in liver and spleen scintigraphy. Initially, thecurcumin-loaded solid lipid nanoparticles (C-SLN) were prepared using microemulsion and solidifcation at lowtemperatures. The size of the C-SLN particle is measured by Laser diffraction (LD) method. Moreover, by applyingthermogravimetric analysis (TGA) it is observed that C-SLN particles are stable with temperature variation. Then,they are radiolabeled with Technetium-99m (99mTc). Using the thin-layer chromatography method, the radiolabelingeffciency was found to be higher than 95%. 99mTc-C-SLNs were injected to the rabbit intravenously (i.v.) andusing a gamma camera, dynamic images were recorded. Liver and spleen were clearly displayed in these dynamicimages. In addition to scintigraphic fndings, the presence of 99mTc-C-SLNs in the liver and the spleen was observedin the ex vivo biodistribution studies and the analyses performed under the fluorescence microscope.
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