Sudaki Pestisit Kalıntılarının Belirlenmesi için GC-NPD Kullanılarak Method Geliştirilmesi ve Validasyonu

Organik fosforlu pestisitlerin ve herbisitlerin sıvı-sıvı ekstraksiyonu (LLE) ve ardından gaz kromatografisiazot-

Anahtar Kelimeler:

Çevre kirliliği, çoklu kalıntı analizleri, GC/NPD, metod validasyonu, pestisit

Method Development and Validation for the Determination of Pesticide Residues in Water by GC-NPD

A multi-residue method was developed for the determination of organophosphorous pesticides andHerbicide by liquid-liquid extraction (LLE) and followed by gas chromatography-nitrogen-phosphorus detector(GC/NPD). The method was evaluated with respect to the limit of detection and quantification, linearity and accuracy(repeatability, reproducibility, recovery). The method is linear over the range 2.5 -25 μg L-1 for eleven pesticidesand 5-25 μg L-1 for methamidophos. Correlation coefficients were higher or equal to 0.992. The limits of detection(LODs) were between 0.67 and 2.23 μg L-1. The limits of quantification (LOQs) ranged from 2.24 and 7.45 μg L-1.Recoveries of fortified water samples in two different concentration levels with 12 organophosphorous pesticidesand herbicide were over 95% in high concentration and 80% in low concentration. For repeatability, relative standarddeviation (RSD%) ranged between 2.79 and 10.99%, and for reproducibility (RSD%) ranged between 1.56and 10.36 %. The developed method is suitable for routine application in water samples in accordance with thevalidation data and the parameter as the high sample throughput and cost effective.

Keywords:

Environmental pollution, GC/NPD, method validation, multi-residue analysis, pesticide,

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