Kadmiyum Klorür Toksisitesinin Karaciğer, Böbrek ve Pankreasta Neden Olduğu Hasarı Melatonin Önler mi?

Yedinci tehlikeli ağır metal olan Kadmiyum, insan sağlığı için temel risk faktörlerinden birisi olarak ifade edilebilir. Besinlerle veya suyla vücuda alınan Kadmiyum, doku ve organlarda birikerek patolojilere yol açar. Düzenleyici görevleri olan Melatonin, aynı zamanda antioksidan bir hormondur. Çalışmanın amacı, oral ve intraperitonal yolla CdCl2 verilen gebe farelerin karaciğer, böbrek ve pankreaslarında belirlenen patolojilerin önlenmesinde melatoninin koruyucu rolünün araştırılmasıdır. Sunulan çalışmada gebelik sürecinde Cd alımı arttığı için gebe fareler kullanıldı. 42 gebe fare ile yedi grup (n:6) oluşturuldu. Kontrol (I.) grubuna oral yolla serum fizyolojik verildi. II. gruba CdCl2, III. gruba Melatoin, IV. gruba CdCl2+Melatoin oral yolla verildi. V. gruba CdCl2, VI. gruba Melatoin, VII. gruba CdCl2+Melatoin intraperitonal yolla verildi. Her iki uygulama yönteminde CdCl2 için 2 mg/kg/bw, Melatonin için 3 mg /kg/bw kullanıldı. Rutin histolojik işlemler gerçekleştirilen karaciğer, böbrek ve pankreas dokuları Hematoksilen-Eozinle boyandı. Işık mikroskobu ve elektron mikroskobu kullanılarak karşılaştırma yapıldı. CdCl2 verilen grupların ortalama organ ağırlıklarında artış belirlenirken, CdCl2+Mel gruplarında ortalama organ ağırlıklarının azaldığı görüldü (p: 0.02). Uygulama gruplarında CdCl2 kaynaklı patolojiler belirlenirken, CdCl2+Mel verilen gruplarda patolojilerin azaldığı belirlendi. İyileşmenin en çok karaciğerde olduğu görüldü. Melatonin’in karaciğer, böbrek ve pankreasta görülen CdCl2 kaynaklı patolojilerin tedavisinde etkili olabileceği belirlendi. Gebelik döneminde kadmiyuma yoğun olarak maruz kalan kadınların, uyku düzenlerine dikkat etmeleri ve melatonin takviyesi almaları önerilir. Kadmiyum maruziyetine karşı melatoninin diğer organlardaki etkisinin belirlenmesi için uzun süreli çalışmalara ihtiyaç olduğu düşünülmektedir.

Anahtar Kelimeler:

Kadmiyum, Melatonin, Toksisite, Gebelik

Does Melatonin Prevent Damage Caused by Cadmium Chloride Toxicity to the Liver, Kidney, ve Pancreas?

Cadmium, the seventh dangerous heavy metal, it may be one of the main risk factors for human health. Cadmium taken into the body with food or water causes pathologies by accumulating in tissues ve organs. Melatonin, which has regulatory functions, is also an antioxidant hormone. The aim of this study is to investigate the protective role of melatonin in the prevention of pathologies determined in the liver, kidney ve pancreas of pregnant mice given CdCl2 via oral ve intraperitonally. Pregnant mice were used because Cd uptake was increased during pregnancy. Seven groups (n:6) were formed with 42 pregnant mice. The saline was given to the control (first) group orally. CdCl2 was given in group II, Melatoin was given Group III, CdCl2+Melatoin was given Group IV orally. CdCl2 was given in group V, Melatoin was given Group VI, CdCl2+Melatoin was given Group VI intraperitoneally. For the both application method 2 mg/kg/bw CdCl2 and 3 mg/kg/bw Melatonin were used. Hematoxylin-Eosin was applied to the tissues for which routine histological procedures were performed. For evaluation used the light and electron microscope. While an increase determined in average organ weights in CdCl2 groups, the average organ weights in CdCl2+Mel groups was decreased. While pathologies were observed in CdCl2 groups, the pathologies decreased in CdCl2+Mel groups. Healing effect of Melatonin was mostly in the liver. Melatonin may be effective to treatment pathologiesons in the liver, kidney and pancreas. Pregnant women exposed to cadmium during should pay attention to their sleep patterns and take melatonin supplements. Long-term studies are needed to determine effect of melatonin in other organs against cadmium exposure.

Keywords:

Cadmium, Melatonin, Toxicity, Pregnancy,

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