Fourier Dönüşümlü Kızılötesi Spektroskopisinin Prostat Kanseri Teşhisinde Kullanılabilirliğinin Araştırılması

Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR) yöntemi, organik ve bazı durumlarda inorganik

Anahtar Kelimeler:

FT-IR Spektroskopisi, kanser teşhisi, kalitatif analiz, prostat kanseri, kanser teşhisi

Investigation of the Usability of Fourier Transform Infrared Spectroscopy in Diagnosis of Prostate Cancer

Fourier Transform-Infrared Spectroscopy (FT-IR) is an analytical technique used to identify inorganic and some cases inorganic materials. This technique measures the absorption of infrared radiation by thesample material versus wavelength. The infrared absorption bands identify molecular components and structures.Prostate cancer is cancer that occurs in the prostate a small walnut shaped gland in men that produces the seminalfluid that nourishes and transports sperm. Prostate cancer is one of the most common types of cancer in men.Prostate cancer that is detected early when it’s still confined to the prostate gland has a better chance of successfultreatment. It is aimed to develop a new alternative chemometrics assisted method to separate and characterizeprostat cancer tumors from healthy cells by simple, cheap and rapid FT-IR method with good accuracy andsensitivity. In order to perform such a study paraffin embedded blocks including both cancer and healthy cellswhich are labelled by the histopathologic measurements were cut to 20 microns thick were located on a microscopeslide and deparafinized. Both healthy (n=10) and cancerous tissues (n=10) were exposured to infrared light betweenwavenumber of 50-4000 cm-1. Orthogonal partial least square analysis (O-PLS) algorithm which is an advancedform of Principle Component Analysis (PCA) was applied to 20 samples to detect their behaviour against infraredlight in between 50-4000 cm-1. Obtained spectrums were evaluated on MATLAB software PLS Toolbox packageprogram. O-PLS analysis were carried out in order to separate cancer and healthy tissues. Sensitivity and specificityof the proposed method is so high with the aid of Orthogonal Signal Correction (OSC) preprocessing method. Asa result, an alternative FT-IR method for the diagnosis of prostate cancer from paraffin blocks has been developedand successfully applied.

Keywords:

FT-IR Spectroscopy, qualitative analysis, prostate cancer, cancer diagnosis,

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