İlknur POLAT, Aytül YILDIRIM, İlker KURBETLİ, Ayşe Serpil KAYA, Uğur KAHRAMAN, Emine GÜMRÜKÇÜ, Köksal AYDİNŞAKİR

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Identification of Races 1, 2, 4 and 8 of Fusarium oxysporum f. sp. dianthi in Turkey by Using Molecular Markers

Fusarium oxysporum f.sp. dianthi (Prill and Delacr.) (Fod) is a causal agent of wilt disease of carnation (Dianthus caryophyflus L.) with its different physiological races. Although eleven physiological races of Fod have been reported in the world, a polymerase chain reaction (PCR)-based diagnostic tool was developed for identification of Fod races 1, 2, 4, and 8, which are the most commonly found in many countries. On the other hand, there is no information about which races are found in Turkey. A total of 91 isolates were collected from plants with Fusarium-like symptoms in the most grown carnation areas in Antalya, İzmir, Isparta and Yalova provinces and all isolates were identified using molecular methods. As a conclusion, the results showed that Fod isolates collected from the most carnation grown provinces of Turkey consist mostly of race 1, 2 and 8. This is the first study reporting Fod races causing wilt disease on carnation plants in Turkey.
Keywords:

Carnation, Fod, Fusarium wilt, PCR, Races,

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  • Arıcı, Ş.E., & Kazaz, S. (2013). Occurrence of carnation root and crown rot under greenhouse condition in Isparta. Reserach Journal of Agricultural Sciences, 6:159-162 (in Turkish).
  • Arıcı, Ş.E., Tuncel, Z.N., & Erdoğan, O. (2018). The effect of some biological preparations against Fusarium wilt of carnation (Fusarium oxysporum f.sp. dianthi). 6th ASM International Congress of Agriculture and Environment Congress, 11-13 October, 2018, Antalya, Turkey.
  • Atakan, A., & Özgönen Özkaya, H. (2020). Determination of Fusarium species in carnation greenhouses in Antalya, Turkey. Communications Faculty of Sciences University of Ankara Series C Biology, 29:131-147.
  • Baayen, R.P., van Dreven, F., Krijger, M.C., & Waalwijk, C. (1997). Genetic diversity in Fusarium oxysporum f. sp. dianthi and Fusarium redolens f. sp. dianthi. European Journal of Plant Pathology, 103:395-408.
  • Baysal, Ö., Siragusa, M., Ikten, H., Polat, I., Gumrukcu, E., Yigit, F., Carimi, F., & Texeira da Silva, J.A. (2009). Fusarium oxysporum f. sp lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey. Physiological and Molecular Plant Pathology, 74:68-75.
  • Ben-Yephet, Y., Reuven, M., Lampel, M., Nitzani, Y., & Mor, Y. (1992). Fusarium oxysporum f. sp. dianthi races in carnation. Phytoparasitica, 20:225.
  • Ben-Yephet, Y., & Shtienberg, D. (1997). Effects of the host, the pathogen, the environment and their interactions, on Fusarium wilt in carnation. Phytoparasitica, 25:207-216.
  • Bogale, M., Wingfield, B.D., Wingfield, M.J., & Steenkamp, E.T. (2007). Species-specific primers for Fusarium redolens and a PCR-RFLP technique to distinguish among three clades of Fusarium oxysporum. FEMS Microbiology Letters, 271:27-32.
  • Castano, R., Sherm, B., & Avilés, M. (2014). Genetic diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain. Journal of Mycology, 2014:1-14.
  • Cer, C. (2021). Occurrence and control measures of Fusarium wilt (Fusarium oxysporum f. sp. dianthi) in carnation growing areas in İzmir province. PhD Thesis, Adnan Menderes University, Aydın.
  • Chiocchetti, A., Bernardo, I., Daboussi, M.J., Garibaldi, A., & Gullino, L. (1999). Detection of Fusarium oxysporum f. sp. dianthi in carnation tissue by PCR amplification of transposon insertions. Phytopathology, 89:1169-1175.
  • Coskuntuna, A., & Yıldız, F. (2006). The biological control of Fusarium wilt on carnation with fluorescent pseudomonads. Journal of Turkish Phytopathology, 35:43-56.
  • Deng, Z. (2018). Breeding for disease resistance in florists’ crops. In: McGovern R, Elmer W (Eds) Handbook of Florists' Crops Diseases. Handbook of Plant Disease Management. Springer, Cham.
  • Garibaldi, A., Lento, G., & Rossi, G. (1986). Il Fusarium oxysporum f. sp. dianthi in Liguria. Indagine sulla diffusione dei diversi patotipi nelle colture di garofano. Panorama Floricolo, 11:1-4.
  • Garibaldi, A., & Gullino, M.L. (1987). Fusarium wilt of carnation: present situation, problems and perspectives. Acta Horticulturae, 216:45-54.
  • Kalc Wright, G.F., Guest, D.I., Wimalajeewa, D.L.S., & van Heeswijk, R. (1996). Characterisation of Fusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay. European Journal of Plant Pathology, 102:451-45.
  • Katan, T., Katan, J., & Hadar, E. (1989). Vegetative compatibility of Fusarium oxysporum f.sp. dianthi from carnation in Israel. Plant Pathology, 38:376-381.
  • Kazaz, S., Kılıç, T., Doğan, E., Mendi, Y., & Karagüzel, Ö. (2020). Süs bitkileri üretiminde mevcut durum ve gelecek. Türkiye Ziraat Mühendisliği Teknik Kongresi Bildiriler Kitabı-1, s: 673-698, (in Turkish).
  • Manulis, S., Kogan, N., Reuven, M., & Ben-Yephet, Y. (1994). Use of the RAPD techniques for identification Fusarium oxysporum f. sp. dianthi from carnation. Phytopathology, 84:98-101.
  • Migheli, Q., Briatore, E., & Garibaldi, A. (1998). Use of random amplified polymorphic DNA (RAPD) to identify races 1, 2, 4 and 8 of Fusarium oxysporum f. sp. dianthi in Italy. European Journal of Plant Pathology, 104:49-57.
  • Polat, İ., Baysal, Ö., Mercati, F., Kitner, M., Cohen, Y., Lebeda, A., & Carimi, F. (2014). Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers. European Journal of Plant Pathology, 139:641-653.
  • Poli, A., Bertetti, D., Rapetti, S., Gullino, M.L., & Garibaldi, A. (2013). Characterization and identification of Colombian isolates of Fusarium oxysporum f. sp. dianthi. Journal of Plant Pathology, 95:255-263.
  • Prados-Ligero, A.M., Basallote-Ureba, M.J., López-Herrera, C.J., & Melero-Vara, J.M. (2007). Evaluation of susceptibility of carnation cultivars to Fusarium wilt and determination of Fusarium oxysporum fsp. dianthi races in Southwest Spain. Hortscience, 42: 596-599.
  • Sarrocco, S., Falaschi, N., Vergara, M., Nicoletti, F., & Vannacci, G. (2007). Use of Fusarium oxysporum f. sp. dianthi transformed with marker genes to follow colonization of carnation roots. Journal of Plant Pathology, 89:47-54.
  • Scovel, G., Ovadis, M., Vainstein ,A., Reuven, M., & Ben-Yephet, Y. (2001). Marker assisted selection for resistance to Fusarium oxysporum in the greenhouse carnation. Acta Horticulture, 552:151-156.
  • Tezcan, H., Karabulut, Ö.A., & İlhan, K. (2004). Investigations on the determination of fungal pathogens causing root and crown rot diseases of cut flowers in Yalova. Journal of Agricultural Faculty of Bursa Uludag University, 18:1-10 (in Turkish).
  • Vergara, M., Sarracco, S., Falaschi, N., Nicoletti, F., & Vannacci, G. (2007). Fusarium oxysporum f. sp. dianthi transformed with marker genes as a tool for studying resistance in dianthus. Acta Phytopathologica et Entomologica Hungarica, 42:297-303.
  • Werner, M., & Irzykowska, L. (2007). The pathogenicity and DNA polymorphism of Fusarium oxysporum originating from Dianthus caryophyllus, Gypsophila spp. and soil. Phytopathologia Polonica, 46:25-36.
  • Zahiri, P., Zamanizadeh, H.R., & Morid, B. (2013). Detection of Fusarium oxysporum f. sp. dianthi races in Iran. Journal of Plant Pathology, 95:69-77.
Horticultural Studies-Cover
  • ISSN: 2717-882X
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2020
  • Yayıncı: Tarım ve Orman Bakanlığı
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