Since the ancient times, it is well known that honey has a therapeutic effects on human health. However, the effectiveness of antibiotics is diminished as resistant pathogens develop and spread. So in this case, we need alternative antimicrobial agents and it is important to use medicinal important materials such as plants, plant based products including honey to struggle this situation. There are too many studies have conducted on antimicrobial activity and researchers have been reported both bacteriostatic and bactericidal effects of honey. The aim of this research is screening the antimicrobial effects of six different honey samples from Kosovo against some gram-positive (Enterobacter faecalis ATCC 29212, Staphylococcus aureus, S. aureus ATCC 29213, S. aureus ATCC 6538P, S. aureus ATCC 25923, Bacillus cereus )and gram-negative (Escherichia coli ATCC 25922, Salmonella tyhimurium ATCC 51812) bacteria by using the agar well diffusion method on Mueller Hinton Agar. According to our results all the honey samples have effectively high antibacterial activity against especially Staphylococcus strains and Salmonella typhimurium ATCC 51812 when comparing the reference antibiotics used in the study.