Funda E. ATAÇ, Turgay TAŞKIN, Erdinç DEMİRÖREN

Sıcaklık stresinin saanen keçilerinde T3, T4, ve kortisol hormon düzeyleri üzerine etkisi

Çalışma, Saanen keçilerinde sıcaklık stresinin etkisini belirlemek amacıyla yapılmıştır. Bu amaçla, stres hormonlarından T3 (triiodothyronine), T4 (thyroxine) ve kortisolun kan örneklerindeki yoğunlukları ölçülmüştür. Ege Üniversitesi Ziraat Fakültesi Zootekni Bölümü’nde yetiştirilen 15 baş Saanen keçi deneme materyali olarak kullanılmıştır. Heparinli tüpler kullanılarak keçilerde boyun toplar damarından alınan kan örnekleri (10 ml), 20 dk süreyle santrifüj (3000 devir/dk) edilmiş ve plazmalar değerlendirmek üzere dondurularak (-200C’de) saklanmıştır. Plazma örneklerindeki T3, T4 ve kortisol konsantrasyonlarını analiz yapmak için RIA kitlerinden yararlanılmıştır (DPC, Coat-A-Count Canine T3, Coat-A-Count Canine T4 ve Coat-A-Count Cortisol, ABD). Saanen keçilerde sıcaklık nem indeksi (SNİ) 79.48 dir. T3, T4, kortisol ve T4/T3 oranına ait ortalamalar sırasıyla; 1.54 (nmol/L), 42.37 (nmol/L), 8.84 (nmol/L) ve 6.38 dir. Sonuç olarak bu çalışmada incelenen hormonlardan sadece T3 ve T4’e ait değerleri yorumlamak yeterlidir. Saanen keçilerinde SNİ, aşırı sıcak stresini ifade etmektedir, ancak T3, T4 ve kortisol hormon düzeylerine göre Saanen keçilerinin sıcaklık stresine uyum gösterdiği söylenebilir. Temel bileşenlerine ayırma analizinde (TBA) incelenen parametre sayısını artırmak ekonomik olmayabilir.

The effect of thermal stress on T3, T4 and cortisol hormanes levels in saanen goats

Study was carried out to determine effect of heat stress in Saanen goats. For this aim, it was determined T3, T4 and cortisol hormone levels in blood samples. Fifteen head Saanen goats, raised in University of Ege, Agriculture Faculty, Department of Animal Science, İzmir were used to be experimental material in this study. Plasma samples were assayed for T3, T4 and cortisol hormone concentrations using a radioimmunoassay (RIA) kit (DPC, Coat-A-Count Canine T3, Coat-A-Count Canine T4 ve Coat-A-Count Cortisol, USA). Blood samples (10 ml) were collected by venapuncture, using heparin as anticoagulant, for 20 minutes by centrifuged (3000 return/min) and the plasma stored frozen (-20oC) until hormones assays were performed. Heat stress index (THI) in Saanen goats was 79.48. T3, T4, cortisol hormone and T4/T3 rate were 1.54 (nmol/L), 42.37 (nmol/L), 8.84 (nmol/L) and 6.38, respectively. Finally, THI has been reflected excess heat stress in Saanen goats, but it can be said Saanen goats have adapted to heat stress. Principle Component Analyses (PCA), it can’t be economic to increase parameters number.

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