Süleyman Mesut PINAR, Zübeyir HUYUT, Saadet BELHAN, Yeter DEĞER

Streptozotosin Kaynaklı Diyabetik Sıçanlarda Diplotaenia Turcica Kök Ekstraktının Sperm Parametreleri ve Üreme Hormonları Üzerine Etkileri

Bu çalışma, Streptozotosin (STZ) kaynaklı diyabetik sıçanlarda Diplotaenia turcica kök ekstraktının sperm parametreleri ve üreme hormonları üzerindeki etkilerini belirlemek amacıyla yapıldı. Çalışma eşit sayıda 6 gruba ayrılan toplam 42 erkek rat üzerinde yapıldı. Grup 1'e intraperitoneal olarak tek doz serum fizyolojik uygulandı. Grup 2'ye intraperitonal yolla STZ 45 mg/kg uygulandı. Grup 3’e 100 mg/kg Diplotaenia turcica kök ekstraktı 28 gün boyunca gastrik gavajla uygulandı. Grup 4’e 200 mg/kg Diplotaenia turcica kök ekstraktı 28 gün boyunca gastrik gavajla uygulandı. STZ 45 mg/kg, Grup 5'e intraperitonal olarak uygulandı. Ek olarak Diplotaenia turcica kök ekstraktı 100 mg/kg, 28 gün boyunca gastrik gavaj yoluyla uygulandı. STZ 45 mg/kg, Grup 6'ya intraperitonal olarak uygulandı. Ek olarak Diplotaenia turcica kök ekstraktı 200 mg/kg, 28 gün boyunca gastrik gavaj yoluyla uygulandı. Üreme hormonları ve sperm parametreleri analiz edildi. Grup 2'de sperm motilitesi ve yoğunluğu ile üreme hormonu değerlerinin Grup 1'e göre anlamlı derecede düşük olduğu ve anormal sperm oranının Grup 1'e göre anlamlı derecede yüksek olduğu belirlendi. Grup 3 ve 4’ün testosteron düzeyleri, Grup 2, 5 ve 6’ya göre oldukça yüksekti. Diyabetli gruba ilave olarak verilen Diplotaenia turcica kök ekstraktının 100 mg/kg’lık dozunun sperm parametrelerinde ve testosteron hormonunda bir iyileştirme oluşturduğu, ancak 200 mg/kg’lık dozunun aynı etkiyi oluşturmadığı tespit edildi. Sonuç olarak, Diplotaenia turcica kök ekstraktının 100 mg/kg dozunu, diyabetli hastalarda önerebiliriz.

Anahtar Kelimeler:

Diplotaenia turcica kök ekstraktı, Diyabet, Erkek Sıçan, Sperm Parametreleri, Üreme hormonları

The Effects of Diplotaenia Turcica Root Extract on Sperm Parameters and Reproductive Hormones in Streptozotocin Induced Diabetic Rats

This study was conducted to determine the effects of Diplotaenia turcica root extract on sperm parameters and reproductive hormones in streptozotocin (STZ) induced diabetic rats. The study was performed on a total of 42 male rats divided into 6 groups of equal numbers. Single-dose physiological saline was administrated intraperitoneally to Group 1. STZ 45 mg/kg was administered intraperitoneally to Group 2. Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days to Group 3. Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days to Group 4. STZ 45 mg/kg administrated intraperitoneally to Group 5. In addition, Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days. STZ 45 mg/kg administrated intraperitoneally to Group 6. Also, Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days. Reproductive hormones and sperm parameters were analyzed. In Group 2, it was determined that sperm motility and density and reproductive hormone values were significantly lower when compared to group 1, and the abnormal sperm rate was significantly higher when compared to Group 1. Testosterone levels in Groups 3 and 4 were significantly higher than Groups 2, 5, and 6. It was determined that the 100 mg/kg dose of Diplotaenia turcica root extract, which is given also to the diabetic group, creates an improvement in sperm parameters and the hormone testosterone, but the 200 mg/kg dose does not have the same effect. As a result, we can recommend the 100 mg/kg dose of Diplotaenia turcica root extract in patients with diabetes.

Keywords:

Diabetes, Diplotaenia turcica root extract, Male rat, Reproductive hormones, Sperm parameters,

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