Bülent ÖZSOY, Altuğ KÜÇÜKGÜL, Nihat YUMUŞAK, Şule Yurdagül ÖZSOY

Deneysel Diabetes Mellitus ve Nonalkolik Karaciğer Yağlanması Oluşturulan Ratlarda Kefirin Koruyucu Etkilerinin Araştırılması

Bu çalışmada kefirin deneysel olarak oluşturulan Tip 2 Diabetes Mellitus (T2DM) ve Nonalkolik Karaciğer Yağlanmasında (NAFLD) karaciğer dokusunda oluşan hasara karşı koruyucu etkileri araştırıldı.Toplam 30 adet Wistar albino ırkı dişi rat kullanıldı. Ratlar her grupta 6 adet olmak üzere 5 gruba ayrıldı. Kontrol grubu hayvanlara herhangi bir uygulama yapılmadı. T2DM oluşturmak amacıyla hayvanlara tek doz 80 mg/kg intraperiteonal Streptozotocin, NAFLD oluşturmak için de hayvanlara yüksek yağlı rat yemi deneme boyunca verildi. Deneme grubundaki hayvanlara sırasıyla yüksek yağlı rat yemi, yüksek yağlı rat yemi+30 ml/kg kefir (oral gavaj), yüksek yağlı rat yemi+Streptozotocin 80 mg/kg intraperiteonal (İP), yüksek yağlı rat yemi+30 ml/kg kefir (oral gavaj)+ Streptozotocin 80 mg/kg (İP) verildi. T2DM oluşturulan gruba kıyasla kan glikoz düzeylerinde kefir eklenmesi ile düşme gözlendi. Ayrıca T2DM ve NAFLD grubunda serumda artan AST, ALT, total protein, kolesterol, trigliserit değerlerinin kefir eklenmesi ile azaldığı gözlendi. Histopatolojik bulgular da biyokimyasal sonuçları desteklemektedir. Kefir eklenen grubun karaciğerlerinde normale yakın histolojik yapı gözlendi. Sonuç olarak karaciğer dokusunda ciddi hasarlara neden olan T2DM ve NAFLD ye karşı kefir içeceğinin tüketilmesinin yararlı olacağı kanısına varıldı.

Anahtar Kelimeler:

Karaciğer, Kefir, Nonalkolik karaciğer yağlanması, Tip 2 diabetes mellitus

Investigation The Protective Effects of Kefir in Experimental Diabetes Mellitus and Nonalcoholic Liver Fattened Rats

In this study the protective effect of kefir against liver tissue damage at experimental Type 2 Diabetes Mellitus (T2DM) and Nonalcoholic Fatty Liver Disease (NAFLD) was investigated. A total of 30 Wistar albino rabbit female rats were used. Rats were divided into 5 groups each group had 6 animals. Any application was done to control group animals. A single dose of 80 mg / kg intraperitoneal Streptozotocin was given to the animals to form T2DM, and a high fat rat was fed to the animals throughout the experiment to generate NAFLD. The animals in the experimental group were fed high fat rat diet, high fat rat diet + 30 ml / kg kefir (oral gavage), high fat rat diet + Streptozotocin 80 mg / kg intraperitoneal (IP), high fat rat diet + 30 ml / kg kefir Oral gavage) + Streptozotocin 80 mg / kg (IP). A decrease in blood glucose levels was observed with the addition of kefir compared to the T2DM group. Also increased serum AST, ALT, total protein, cholesterol, triglyceride levels were decreased by the addition of kefir in the T2DM and NAFLD groups. Histopathological findings also support biochemical results. In the liver of kefir-added group, close to normal histological structure was observed. As a result, it was concluded that consumption of kefir beverage would be beneficial against T2DM and NAFLD, which cause serious damage to the liver.

Keywords:

Liver, Kefir, Nonalcolic fatty liver disease, Type 2 diabetes mellitus,

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