Bazı kaba yemlere ilave edilen probiyotiklerin in vitro organik madde sindirimi ve metan üretimi üzerine etkileri

Bu çalışma ruminantlarda yaygın olarak kullanılan bazı kaba yemlere katılan probiyotiklerin (Lactobacillus rhamnosus, Bifidobacterium lactis ve Saccharomyces boulardii) in vitro ortamda metan gazı oluşumuna etkisini tespit etmek için yapılmıştır. Bu amaçla %0.1 oranında probiyotik ilave edilen öğütülmüş kaba yem örnekleri rumen sıvısı içeren özel cam tüpler içerisinde 39 °C’de 24 saat inkube edilmiştir. İnkubasyon sonunda oluşan toplam gaz içerisindeki metan (CH4) gazı ve karbondioksit (CO2) yüzdesi CH4 ölçüm cihazı yardımıyla belirlenmiştir. Ayrıca her bir deneme grubundaki in vitro organik madde sindirilebilirliği (IVOMS), amonyak azotu miktarı (NH3-N), metabolik enerji (ME) ve pH değerleri belirlenmiştir. Buğday samanına ilave edilen B. lactis’in oluşan toplam gaz, CH4 ve CO2 hacmini ve IVOMS’ni düşürdüğü, S. boulardii’nin ise CH4 yüzdesini yükseltirken, CO2 yüzdesini düşürdüğü gözlenmiştir. Çayır kuru otuna ilave edilen L. rhamnosus oluşan toplam gaz miktarını, CH4 miktarını ve IVOMS’ni yükseltirken CH4 yüzdesini etkilememiştir. Silaj ve yonca kuru otuna ilave edilen probiyotikler CH4 ve CO2 düzeylerini etkilememiştir. Sonuç olarak çalışmada buğday samanına katılan B. lactis dışındaki probiyotik mikroorganizmalar CH4 üretimini arttırmış ya da etkilememiştir. Buğday samanına ilave edilen B. lactis’in CH4 miktarını azaltması, Çayır kuru otuna ilave edilen L. rhamnosus’un ise CH4 miktarını arttırmasının söz konusu yemlerin IVOMS’ni etkilemesinden kaynaklandığı, bu nedenle sunulan çalışmada kullanılan probiyotiklerin CH4 miktarını azaltmakta etkili olmadığı sonucuna varılmıştır.

Anahtar Kelimeler:

Probiyotik, Kaba yem, in vitro, Metan üretimi

Effects of Probiotics Added to Some Roughages on in vitro Organic Matter Digestibility and Methane Production

The objective of this study was to determine the effects of probiotics (Lactobacillus rhamnosus, Bifidobacterium lactis and Saccharomyces boulardii) added to some roughages on in vitro methane production. Ground roughage samples added with 0.1% of probiotics were incubated in special glass tubes containing rumen fluid at 39 °C for 24 h. Percentages of methane (CH4) and carbon dioxide (CO2) in total gas produced were measured by using a methane measuring device. Additionally, in vitro organic matter digestibility (IVOMD), amount of ammonia nitrogen (NH3-N), metabolic energy (ME) and pH values were also determined. Addition of B. lactis to wheat straw decreased CH4 and CO2 amount and IVOMD while S. boulardii increased the percentage of CH4 and decreased the percentage of CO2. Among probiotics added to grass hay L. rhamnosus increased total gas volume, CH4 volume and IVOMD while it did not affect the percentage of CH4. It was detected that addition of the probiotics to corn silage and alfa alfa roughage did not affect methane and carbondioxyde levels. As a result, except for B. lactis, added to wheat straw, probiotic microorganisms used in the present study increased or did not affect in vitro methane production. The results suggested that decreased methane production in wheat straw added with B. lactis and increased methane production in grass hay added with L. rhamnosus resulted from the affected IVOMD of these roughages due to addition of these probiotics and therefore use of the probiotics in this study was not effective for reducing ruminal methane production.

Keywords:

Probiotics, Roughage, in vitro, Methane production,

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