Topikal D Vitamininin Titanyum İmplantların Osseointegrasyonu Üzerine Etkilerinin Değerlendirilmesi: Deneysel Bir Çalışma

Amaç: Bu çalışmanın amacı, titanyum implantların yüzeyine uygulandığında topikal D vitamininin osseointegrasyon üzerindeki etkilerini değerlendirmektir. Materyal ve metod: Bu çalışmada 4 haftalık bir deney periyodunda 20 erkek Spraque-Dawley sıçanı kullanıldı. 4 rat deneyler sırasında öldü. Daha sonra ratlar 2 gruba ayrıldı: Test grubu (n=8) ve kontrol grubu (n=8). Patella diseksiyonu sonrası sıçan femurlarının metafiz kısmına 16 tane kumlanmış ve asitle pürüzlendirilmiş implant cerrahi olarak entegre edildi. Entegrasyon öncesinde 3,2 mm çapında ve 8 mm uzunluğunda olan implantlar, test grubunda % 10'luk bir D vitamini çözeltisine daldırıldı. Kontrol grubuna herhangi bir tedavi uygulanmadı. 4 haftalık deney süresinden sonra, sıçanlar öldürüldü ve implantlar çevreleyen kemik dokusuyla birlikte çıkarıldı. Örnekler % 10 formaldehit solüsyonunda 7 gün fikse edildi. Kemik-implant kaynaşması (BIC) oranları, dekalsifiye edilmemiş histolojik yöntemler kullanılarak belirlendi. Bulgular: Deneyde 2 örnek uygun olmadığı için çıkartıldı.Test (n=7) ve kontrol grubu (n=7) implantları arasında BIC oranları için istatistiksel olarak önemli farklılıklar tespit edilmedi (P> 0.05). Sonuç: Bu hayvan çalışmasının sınırları dahilinde, implant yüzeylerine topikal D vitamini uygulamasının BIC oranlarını etkilemediği sonucuna varıldı.

Anahtar Kelimeler:

osseointegrasyon, kemik-implant kaynaşması, topikal vitami-d, sıçan femoral kemiği

Evaluation of the Effects of Topical Vitamin D on Osseointegration of Titanium Implants: An Experimental Study

Background: The aim of this study was to evaluate the effects of topical vitamin D to on osseointegration when applied to the surface of titanium implants. Material and Metods: Twenty male Spraque-Dawley rats were used in this study in a 4-week experimental period. 4 rats died during the experiments. The rats were divided into 2 groups: a test group and control group. Sixteen sandblasted and acid-etched implants were integrated surgically in the metaphyseal part of rat femurs after patella dissection. Prior to integration, the implants, which were 3.2 mm in diameter and 8 mm in length, were submerged in a 10% vitamin D solution in the test group. No treatment was applied in the control group. After the 4-week experimental period, the rats were sacrificed, and the implants were removed with surrounding bone tissue. Specimens were fixed for 7 days in a 10% formaldehyde solution. Bone implant contact (BIC) ratios were determined after using non-decalcified histological methods. Results: In the experiment, 2 samples were removed because they were not suitable. No statistically significant differences were found for BIC ratios between the test (n = 7) and control group (n = 7) implants (P> 0.05). Conclusion: It was concluded, within the limitations of this animal study, that topical vitamin D application on implant surfaces does not affect BIC ratios.

Keywords:

osseointegration, bone implant contact, topical vitamin-d, rat femoral bone,

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