Seröz vücut sıvılarının akım sitometri ile incelenmesi: Tek merkez deneyimi
Giriş: Güncel pratikte seröz vücut sıvıları biyokimyasal, mikrobiyolojik ve sitomorfolojik incelemeler ile
değerlendirilmektedir. Seröz vücut sıvıları infeksiyöz ve inflamatuar hastalıkların yanı sıra hematopoetik ve
solid neoplastik hastalıklar sonucu da oluşabilmektedir. Hematopoietik hücrelerin immünfenotipik olarak
hızlı değerlendirilmesinde kullanılan akım sitometri yöntemi, özellikle nedeni bilinmeyen seröz vücut
sıvılarının irdelenmesinde alternatif bir tanı yöntemidir. Bu çalışmada 4 yıllık süreçte, seröz vücut
sıvılarının irdelenmesinde akım sitometri deneyimimizi gözden geçirdik.
Materyal ve Metod: Mayıs 2010 - Şubat 2014 tarihleri arasında hastanemiz özel hematoloji bölümü
laboratuvarında altı-renkli akım sitometri cihazı ile analizi yapılmış olan 77 hastanın (40 kadın, 37 erkek,
ortanca yaş: 56 (20-84)), 80 seröz vücut sıvı örneği (45 plevra, 35 periton) retrospektif olarak incelendi.
Bulgular: Kırkbeş plevra sıvı örneğinden 5'inde, 35 periton sıvı örneğinden ise 4'ünde hematolojik neoplazi
için tanısal immünfenotipik bulgular gözlendi. Bu olgularda tanı ve alt tip tayini doku biyopsisi ve/veya
sitomorfolojik analiz ile doğrulandı.
Tartışma: Özellikle agresif seyreden ve zamanın hayati değer taşıdığı hematolojik kanserlerde patolojik
değerlendirme nispeten uzun sürebilmekteyken akım sitometri ile seröz vücut sıvılarının immünfenotipik
analizi daha erken tanıya ulaşmayı sağlayabilir kanısındayız.
Investigation of serous body fluids with flow cytometry: single center experience
Introduction: In current clinical practice serous effusions are evaluated by cytomorphological, biochemical
and microbiological studies. As well as infections and inflammatory diseases, serous body fluids can also be
observed in hematopoietic and solid neoplastic diseases. Flow cytometry (FC) which can be used for
evaluation of hematopoietic cells rapidly is an alternative diagnostic tool to examine unexplained serous
body effusions. In this study, we reviewed our experience of flow cytometry for evaluating the serous body
fluids during 4 years period.
Materials and Methods: From May 2010 to February 2014, a total of 80 consecutive serous effusion specimens (45 pleural, 35 peritoneal) of 77 cases (40 female, 37 male, median age: 56 (20-84)), that were
analysed by the six-color FC in the division of hematology laboratory at Akdeniz University Hospital, were
investigated retrospectively.
Results: Five of 45 pleural fluid samples and 4 of 35 peritoneal fluid samples were observed diagnostic
immunphenotypic findings for hematologic neoplasia. Diagnosis was verified by tissue biopsy and/or
cytomorphological analysis in these cases.
Discussion: Cytopathologic evaluation may be delay especially in the diagnosis of aggressive lymphomas
which requires urgent evaluation and treatment. In this case, immunophenotypic analysis of serous body
fluids by FC can provide to access early diagnosis and also accurate clinical staging.
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