Sıçanlarda Tıkanma Sarılığında Melatonin ve N-Asetilsisteinin Etkileri

Amaç: Tıkanma sarılıklı sıçanlarda, koletastazda N–asetilsistein ve melatoninin etkilerini ve karaciğer ve böbrek hasarında koruyucu rollerini araştırmak ve karşılaştırmak amaçlandı. Materyal ve Metod: 48 sıçan çalışma içinkullanıldı. Sıçanlar sham, ana kontrol ve çalışma olarak 3 ana gruba ayrıldı. Daha sonra ana kontrol grubu erken sakrifikasyon grubu ve kontrol grubuna ayrıldı. Çalışma grupları kendi içinde melatonin, N–asetilsistein ve melatonin & N–asetilsistein olmak üzere üç ayrı alt gruba ayrıldı. Çalışma ve kontrol gruplarında laparotomi yapıldı, koledok dönüldü, bağlandı ve kesildi. İşlemden 5 gün sonra erken sakrifikasyon grubunda; kan örnekleri, karaciğer ve böbrek dokuları alındı. Çalışma gruplarında beşinci günden başlayarak 10. güne kadar subkutan olarak melatonin, N–asetilsistein ve melatonin & N–asetilsistein uygulandı, sham ve kontrol gruplarına serum fizyolojik verildi. 10. Günde tüm gruplardan kan örnekleri, karaciğer ve böbrek doku örnekleri alındı. Bulgular: Total bilirübin, AST, ALT, kreatinin seviyeleri sarılıklı sıçanlarda anlamlı olarak yüksekti. 10. günde kontrol gruplarında AST, ALT, total bilirubin, BUN, kreatinin seviyeleri anlamlı olarak yüksekti. Melatonin, N–asetilsistein ve melatonin & N–asetilsistein grupları karşılaştırıldığında tüm biyokimyasal parametrelerin seviyelerinde anlamlı fark saptanmadı. Kontrol grubunda MDA ve NO seviyeleri çalışma grubundan daha yüksek saptandı. Melatonin, N–asetilsistein ve melatonin & N–asetilsistein grupları karşılaştırıldığında MDA ve NO seviyeleri açısından farklılık yoktu. Histopatolojik bulgularda MDA ve NO değerleri ile benzerlik gösteriyordu. Sonuç: Tıkanma sarılıklı sıçanlarda melatonin ve NAC kullanılması karaciğer ve böbrek dokusunda serbest oksijen radikallerinin yarattığı hasardan korur.

The Effects of Melatonin and N-Acetylcysteine on Obstructive Jaundiced Rats

Aim: The aim of this experimental study is to investigate the effects of N- acetylcysteine and melatoninin on the cholestasis and their protective effects on liver and renal injury. Materials and Methods: Forty eight rats were used in the study. Rats were divided into three main groups as sham, main control, and study groups. Main control group divided early sacrification group, control group, study group is divided melatonin, N-acetylcysteine and melatonin & N-acetylcysteine groups. In study and control groups, a laparotomy was performed and the common bile duct was ligated and divided. Five days after the first operation, from early sacrification group blood samples, and liver and renal tissues were collected. For study groups from the fifth day to day 10 melatonin, N-acetylcysteine, melatonin & N-acetylcysteine solutions were applied subcutaneous, saline to the sham and control group. At the day ten from the all groups blood samples, and liver tissues and renal tissues were collected. Results: AST, ALT, BUN, creatinine, total bilirubin levels were significantly higher in jaundiced rats than sham group rats. AST, ALT, total bilirubin, BUN, creatinin levels were significantly higher in the control group at the end of day 10. Among the melatonin group, N-acetylcyteine group and melatonin & N-acetylcysteine group all biochemical parametres were not different. Also the values of MDA and NO for control group were higher than study groups. On the other hand, MDA and NO values were not different between the melatonin group, N-acetylcyteine group and melatonin & N- acetylcysteine group at the end study. Histopathologic findings were also similiar as NO and MDA values. Conclusion: Use of melatonin and N-acetylcysteine in obstructive jaundiced rats, prevents damages of free oxygen radicals on liver and renal tissue.

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