The Determination of Antioxidant Vitamin Levels, Radical Scavenging Effect of Elaeagnus Angustifolia Methanol Extracts Against to MDA

Free radical; atomic or molecular structures with single-electron compounds. With other molecules that can be very easy to electron exchange across the molecules “an oxidant molecules” or “reactive oxygen particles” also be said. Antioxidants compound that stop the formation of the free radicals or dispel negative effects of items. this, to do inhibited the radical reaction systems. or non-toxic products there is no turning it on. The most of antioxidants found in foods. With an antioxidant-rich diet, free radicals and active oxygen consisting of the which is the basis of disease oxidative damage can be prevented [1]. This the nutrition-type especially fruits vegetables natural anti-oxidant resources [2]. In this study aimed that to determine the Antioxidant vitamin levels, radical-scavenging properties of Elaeagnus angustifolia methanol extracts against to prevent formation MDA. The material obtained from a plant seller in Elazığ. Extracted from plants was performed using methanol solvent. Extraction process was done of plant parts by weight of 2 grams in weight was 10 times the solvents. Spectral analysis with Shimadzu model UV 1400 spectrophotometer, antioxidant vitamins with the same brand HPLC was carried out. Plant extracts of the DPPH radical knockdown activity the method specified,[3] the OHradical destruction the method indicated[4], Antioxidant capacity ABTS radical destruction activity[5] lipid peroxidation (LPO) measurements using as standard Fenton Quercetin, Resvesterol reagents MDA-TBARS by the method[6], vitamins analysis using the methods [7, 8, 9] was performed. Radical scavenging activity from 100μL-800μL concentration measurements were performed with two evil-fold increase. Results of DPPH radical scavenging and OH· radical knock down activity increased with concentration difference. MDA analysis comparison with the control group showed significant differences. MDA analysis with controls Quercetin standards compared were not statistical different, both the extracted measurements and control groups fenton reagent and resveratrol standard compared with a significant statistical difference was observed. p

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1. B.Öztürk-Demirata Antioksidanlar, Proje Danışmanlığı Eğitimi Çalıştayı Çanakkale. http://maycalistayleri. comu.edu.tr. [Access date: 04.02.2014].
2. N. Koca, F. Karadeniz, Natural antioxidant compounds in foods, Food journal, 30 (2005) 229-236.
3. K. Shimada, K. Fujikawa, K. Yahara, T. Nakamura, Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion, Journal of Agricultural and Food Chemistry, 40 (1992) 945-948.
4. B. Halliwell, JMC. Gutteridge, O. Aruom,a The deoxyribose method: a simple “test tube” assay for determination of rate constants for reactions of hydroxyl radicals, Analytical Biochemistry, 165 (1987) 215–219.
5. K Shimoi et all., Radio protective effect of antioxidative flavonoids in γ-ray-irradiated mice, Carcinogenesis, 15 (1994) 2669-2672.
6. L. Wu, L. Chang, S.Chen, N. Fan, J.A Ho, Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive, LWT-Food Science and Technology, 42 (2000) 91513.
7. DI. Sánchez-Machado, J. López-Hernández, P. PaseiroLosada, High performance liquid chromatographic determination of alpha-tocopherol in macro algae, Journal of Chromatography A, 976 (2002) 277.
8. J. Lopez-Cervantes, et all., High performance liquid chromatography method for the simultaneous quantification of retinol, alpha-tocopherol, and cholesterol in shrimp waste hydrolysate, Journal of Chromatography A, 1105 ( 2006) 135.
9. M. Karatepe, Simultaneous Determination of Ascorbic Acid and Free Malondialdehyde in Human Serum by HPLC/UV. LC-GC North America, 22 (2004) 362-365.
10. Z. Okçu, F. Keleş Cardiovascular Diseases and Antioxidants, Journal of Ataturk University the Faculty of Agriculture, (2009).
11. P. Storz, Reactive oxygen species in tumor progression, Front Biosci., 1 (2005) 1881.
12. L. Avi, et al. Oxidative Stress in Parkinson’s Disease, The Open Pathology Journal, 3 (2009) 38-42
13. K.G. Manton et al. ROS effects on neurodegeneration in Alzheimer’s disease and related disorders: on environmental stresses of ionizing radiation, Curr Alzheimer Res, 1 (2004) 277-93.
14. J. Van-Horssen, M.E. Witte, G. Schreibelt, H.E. de Vries , Radical changes in multiple sclerosis pathogenesis, Biochim Biophys Acta, 1812 (2011) 141-50.
15. M.A. Babizhayev Y.E. Yegorov, Reactive Oxygen Species and the Aging Eye: 2010 Specific Role of Metabolically Active Mitochondria in Maintaining Lens Function and in the Initiation of the Oxidation-Induced Maturity Onset Cataract-A Novel Platform of Mitochondria-Targeted Antioxidants With Broad Therapeutic Potential for Redox Regulation and Detoxification of Oxidants in Eye Diseases, Oct 22. [Epub ahead of print].
16. C.L. Si, et al., Antioxidant flavonoids from bark of Elaeagnus angustifolia, Planta Med, 75 (2009)
17. J. Nordbeg, E.S.J. Arner, Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. J. Free Rad.Biol Med, 31( 2001) 1287.
18. W.A. Pryor, J.P. Stanley, A suggested mechanism for the production of malondialdehyde during the autoxidation of polyunsaturated fatty acids, Nonenzymatic production of prostaglandin endoperoxides during autoxidation, J. Org. Chem,. 40(1975) 3615.