Satureja boissieri Uçucu Yağının Antimikrobiyal, Antiproliferatif ve Antioksidan Özelliklerinin Araştırılması

B u çalışmanın amacı Satureja boisiseri Hausskn. ex Boiss. Lamiaceae uçucu yağının biyolojik aktivitelerinin araştırılmasıdır. Uçucu yağın büyük bileşiklerinin antimikrobiyal aktivitesi agar kuyu ve disk difüzyon yöntemleri ile belirlenmiştir. Uçucu yağın, insan kolorektal adenokarsinom HT-29 hücre hattına karşı antiproliferatif etkisi Gerçek Zamanlı Hücre Analizörü xCELLigence yöntemi ile değerlendirilmiştir. Ayrıca, uçucu yağın toplam fenolik miktarı ve radikal giderim aktivitesi de tespit edilmiştir. Sonuçlar göstermektedir ki; timol, %40 timol : %60 karvakrol, %25 terpinen-4-ol: %75 karvakrol S. aureus ATCC 29213 suşlarına karşı önemli bir antimikrobiyal aktiviteye sahiptir. Bununla birlikte, uçucu yağ HT-29 hücre hattının büyümesi üzerine yüksek bir inhibisyon etki göstermiştir IC50

Anahtar Kelimeler:

Satureja boissieri, antimikrobiyal, antiproliferatif, HT-29

Investigation of Antimicrobial, Antiproliferative and Antioxidant Properties of Satureja boissieri Essential Oil

The aim of this study was to investigate the biological activities of Satureja boissieri Hausskn. ex Boiss. Lami- aceae essential oil. Antimicrobial effects of the major components from the essential oil were determined by agar well and disc diffusion methods. Antiproliferative effect of the essential oil against human colorectal adenocarcinoma HT-29 cell line was evaluated by Real Time Cell Analyzer xCELLigence method. In addition, total phenolic content and radical scavenging activity of the essential oil were determined. The results show that thymol, thymol 40%:carvacrol 60%, and terpinene-4-ol 25%:carvacrol 75% had significant antimicrobial activity against S. aureus ATCC 25923 and S. aureus ATCC 29213 strains. Also, the essential oil exhibited the high inhibitory effect on the growth of HT-29 cell line IC50

Keywords:

Satureja boissieri, Antimicrobial, antiproliferative, HT-29,

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