Preparation of Fe(III)-chelated poly (HEMA-MAH) cryogel for lysozyme adsorption

Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [poly(HEMA-MAH)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-50 μm size. Then the monolithic cryogel was loaded with Fe(III) ions to form the metal chelate with poly(HEMAMAH) cryogel. Poly(HEMA-MAH) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-MAH) monolithic cryogel was 2.82 g H2O/g cryogel. Poly(HEMA-MAH) cryogel containing 36.4 $mu mol$ MAH/g was used in the adsorption/desorption of lysozyme from aqueous solutions. The nonspecific adsorption of lysozyme was very low (1.1 mg/g). The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the poly(HEMA-MAH) cyogel without significant loss of adsorption capacity.

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