Ebru AKDOĞAN

Poly-3-hydroxybutyrate ve Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Yüzeylerine Protein Adsorpsiyonu: Adsopsiyon İzotermleri ve Adsorbe Proteinin Konformasyonel Analizi

Sığır serum albümininin (BSA) poli-3-hidroksibutirat (PHB) ve poli(3-hidroksibutirat-co-3-hidroksivalerat) (PHBV) yüzeyleri üzerinde denge adsorpsiyon davranışı farklı çözelti konsantrasyonlarında incelenmiştir. Denge adsorpsiyon verileri, Langmuir ve Freundlich adsorpsiyon modellerine uyarlanmıştır. BSA’nın PHB ve PHBV yüzeylerinde adsorpsiyon davranışı ayrıca Fourier transform kızılötesi (FTIR) spektroskopisisiyle zayıflatılmış toplam yansıma (ATR) aparatı kullanılarak yüzey adsorbe olmuş proteinin konformasyonun analiz edilmesi suretiyle araştırılmıştır. Sonuçlar Freundlich izoterminin, BSA'nın PHB ve PHBV yüzeyleri üzerinde adsorpsiyonunu açıklamak için daha uygun olduğunu göstermiş olup bu durum BSA'nın yüzey adsorpsiyonu sonucu geçirdiği konformasyonel değişikliklerle de desteklenmiştir.

Protein Adsorption on Poly-3-hydroxybutyrate and Poly(3-hydroxybutyrate-co- 3-hydroxyvalerate): Adsorption Isotherms and Conformational Analysis of the Adsorbed Protein

The equilibrium adsorption of bovine serum albumin (BSA) on poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrateco- 3-hydroxyvalerate) (PHBV) surfaces was studied at different solution concentrations. The equilibrium adsorption data were fitted to the Langmuir and Freundlich adsorption models. Adsorption behaviour of BSA on PHB and PHBV surfaces was further investigated by analysing the conformation of the surface adsorbed protein with Fourier transform infrared (FTIR) spectroscopy with an Attenuated Total Reflectance (ATR) apparatus. The results showed that the Freundlich isotherm was a better fit for the adsorption of BSA on PHB and PHBV surfaces, which is supported by the significant conformational changes that BSA undergoes upon adsorption.

PDF

___

1. M. Alkan, Ö. Demirbaş, M. Doğan, O. Arslan, Surface properties of bovine serum albumin–adsorbed oxides: Adsorption, adsorption kinetics and electrokinetic properties, Micropor. Mesopor. Mat., 96 (2006) 331-340.
2. E. Akdoğan, M. Mutlu, Generation of amphoteric surfaces via glow-discharge technique with single precursor and the behavior of bovine serum albumin at the surface, Colloids Surf. B, 89 (2012) 289-294.
3. L.A. Buchanan, A. El-Ghannam, Effect of bioactive glass crystallization on the conformation and bioactivity of adsorbed proteins, J. Biomed. Mater. Res. A, 93 (2010) 537- 546.
4. R.A. Latour, The Langmuir isotherm: a commonly applied but misleading approach for the analysis of protein adsorption behavior, J. Biomed. Mater. Res. A, 103 (2015) 949-958.
5. A.P., Bonartsev, S.G. Yakovlev, I.I. Zharkova, A.P. Boskhomdzhiev, D.V. Bagrov, V.L. Myshkina, T.K. Makhina, E.P. Kharitonova, O.V. Samsonova, A.V. Feofanov, Cell attachment on poly (3-hydroxybutyrate)-poly (ethylene glycol) copolymer produced by Azotobacter chroococcum 7B, BMC Biochem., 14 (2013) 1-13.
6. Y.W. Wang, Q. Wu, G.Q. Chen, Attachment, proliferation and differentiation of osteoblasts on random biopolyester poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds, Biomaterials, 25 (2004) 669-675.
7. R.H. Mendonca, T. de Oliveira Meiga, M.F. da Costa, R.M. da Silva Moreira Thire, Production of 3D scaffolds applied to tissue engineering using chitosan swelling as a porogenic agent, J. Appl. Polym. Sci., 129 (2013) 614-625.
8. C. Lee, M. Horiike, K. Masutani, Y. Kimura, Characteristic cell adhesion behaviors on various derivatives of poly (3-hydroxybutyrate) (PHB) and a block copolymer of poly (3-[RS]-hydroxybutyrate) and poly (oxyethylene), Polym. Degrad. Stab., 111 (2015) 194-202.
9. A. Bonartsev, V.V. Voinova, E.S. Kuznetsova, I.I. Zharkova, T.K. Makhina, V.L. Myshkina, D.V. Chesnokova, K.S. Kudryashova, A.V. Feofanov, K.V. Shaitan, BSA adsorption on porous scaffolds prepared from bBioPEGylated poly (3-Hydroxybutyrate), Appl. Biochem. Microbiol., 54 (2018) 379-386.
10. L. Rouxhet, R. Legras, Y.J. Schneider. Interactions between a biodegradable polymer, poly (hydroxybutyratehydroxyvalerate), proteins and macrophages. in Macromol. Symp., 130 (1998) 347-366.
11. Z. Zheng, F.F. Bei, H.L. Tian, G.Q. Chen, Effects of crystallization of polyhydroxyalkanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes, Biomaterials, 26 (2005) 3537-3548.
12. M. Domínguez-Díaz, A. Meneses-Acosta, A. Romo-Uribe, Adhesion and cell viability of normal human osteoblasts (NHOst) on scaffolds of poly (3-hydroxybutyrate), MRS Onl. Proceed. Lib. (OPL), 1721 (2015) 20-26.
13. I. Langmuir, The adsorption of gases on plane surfaces of glass, mica and platinum, J. Am. Chem. Soc., 40 (1918) 1361- 1403.
14. H. Freundlich, Over the adsorption in solution, J. Phys. Chem, 57 (1906) 1100-1107.
15. C. Ng, J.N. Losso, W.E. Marshall, R.M. Rao, M. Ramu, Freundlich adsorption isotherms of agricultural by-productbased powdered activated carbons in a geosmin–water system, Bioresour. Technol., 85 (2002) 131-135.
16. C.M. Alves, R.L. Reis, J.A. Hunt, The competitive adsorption of human proteins onto natural-based biomaterials, J. R. Soc. Interface, 7 (2010) 1367-1377.
17. A.O. Pérez-Arauz, A.E. Aguilar-Rabiela, A. Vargas-Torres, A.I. Rodríguez-Hernández, N. Chavarría-Hernández, B. Vergara- Porras, M.R. López-Cuellar, Production and characterization of biodegradable films of a novel polyhydroxyalkanoate (PHA) synthesized from peanut oil, Food Packag. Shelf Life, 20 (2019) 100297-100305.
18. K.P. Ishida, P.R. Griffiths, Comparison of the amide I/II intensity ratio of solution and solid-state proteins sampled by transmission, attenuated total reflectance, and diffuse reflectance spectrometry, Appl. Spectrosc., 47 (1993) 584- 589.
19. T.J. Lenk, B.D. Ratner, R.M. Gendreau, K.K. Chittur, IR spectral changes of bovine serum albumin upon surface adsorption, J. Biomed. Mater. Res., 23 (1989) 549-569.
20. V. Della Porta, E. Bramanti, B. Campanella, M.R. Tiné, C. Duce, Conformational analysis of bovine serum albumin adsorbed on halloysite nanotubes and kaolinite: A Fourier transform infrared spectroscopy study. RSC Adv., 6 (2016) 72386-72398.
21. B. Stuart, Infrared spectroscopy, John Wiley & Sons, New York, USA, 2004.
22. P. Roach, D. Farrar, C.C. Perry, Interpretation of protein adsorption: surface-induced conformational changes. J .Am. Chem. Soc., 127 (2005) 8168-8173.
23. Y. Jeyachandran, E. Mielczarski, B. J.A. Mielczarski, Quantitative and qualitative evaluation of adsorption/ desorption of bovine serum albumin on hydrophilic and hydrophobic surfaces, Langmuir, 25 (2009) 11614-11620.
24. K. Zhou, J. Chen, T. Wang, S. Yanjing, L. Qiao, Y. Yu, Effect of surface energy on protein adsorption behaviours of treated CoCrMo alloy surfaces, Appl. Surf. Sci., 520 (2020) 146354- 146366.