Ramazan Demirdağ, Ahmet Özkaya, Murat Koca, Zafer Şahin, Müslüm Kuzu, Veysel Çomaklı, Ugur Dardağan, Kader Uzun

$H_2O_2$ Uygulanan Sıçanlarda Yeni Karbonik Anhidraz Aktivatörleri

Karbonik anhidrazın pH düzenlenmesi, $HCO_3$-geri emilimi, $CO_2$’in solunumla atılması gibi görevlerinin yanısısıra, hafıza, sinyal prosesleri, uzun dönem sinaptik transformasyon olaylarında önemli rolleri vardır. Yapılan çalışmada, sentetik ürünler olan (Benzofuran-2-il)(3-fenil-3-metilsiklobutil) ketoksim (A), (Benzofuran2-il)(3-metil-3-mezitilsiklobutil)keton tiyosemikarbazon (T), 1,3-bis(2-klorobenzoil)imidazolin-2-tiyon (B) ve $H_2O_2$’in karbonik anhidraz aktivitesi üzerine erkek Wistar karaciğer, eritrosit, kalp ve böbrek dokularında etkileri incelendi. Bu amaçla, kontrol grubu, her bir sentetik ürün için bir grup, $H_2O_2$ grubu ve sentetik ürünler ile $H_2O_2$ kombinasyon grupları oluşturuldu. Elde edilen verilerden $H_2O_2$ uygulan grupta bütün dokularda karbonik anhidraz aktivitesinde azalma olduğu görüldü. Bununla birlikte A maddesi karaciğerde aktivasyona neden olurken T maddesi eritrosit ve kalp dokularında, B maddesi ise karaciğer ve eritrositlerde aynı etkiyi gösterdi.

Novel Carbonic Anhydrase Activators in the Rats Exposed to $H_2O_2$

Carbonic anhydrase has an important role in attentional gating of memory storage, signal process and longterm synaptic transformation beside its pH regulation, $HCO_3$-reabsorption and $CO_2$ expiration tasks. Within the study, in vivo effects of (Benzofurane-2-yl)(3-phenyl-3-methylcyclobutyl)ketoxime (A), (Benzofurane-2-yl) (3-methyl-3-mesitylcyclobutyl)ketone thiosemicarbazone (T), 1,3-bis(2-chlorbenzoyl)imidazoline-2-thione (B) which are synthetic products and $H_2O_2$ (H) on the carbonic anhydrase activity in the liver, erythrocyte, heart and kidney tissues of male Wistar rat were analyzed. For this purpose, control group, separate groups for each of the synthetic products, $H_2O_2$ group and $H_2O_2$ combination group with synthetic products were created. As a result of the obtained evidence, a decrease in carbonic anhydrase activity was observed in examined tissues exposed to $H_2O_2$, but A compound has activation effect on enzyme activity in liver, T compound has it in erythrocyte and heart tissues, B compound has it in liver and erythrocytes.

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