Hüseyin CAN, Seçkin SOYA, Mehmet Salih YIKILMAZ

Bombyx mori’nin Larval Testislerinden Elde Edilen Yüzeye Bağımlı ve Süspanse Lepidopter Hücrelerinin Primer Kültürü

Ç alışmada öncelikli olarak Bombyx mori’nin larval testislerinden primer böcek hücre kültürü oluşturulması amaçlanmıştır. Diğer bir yandan yetiştirilen bu hücrelerin sürdürülebilir hücre hatları oluşturma potansiyelleri araştırılmıştır. Testisler Bombyx mori’nin 5.evre larvalarından elde edilmiş ve Grace insect medium’da kültüre edilmiştir. Sonuç olarak, Bombyx mori 5.evre larva testislerinden elde edilmiş primer böcek hücre kültüründe hücrelerin süspanse ve yüzeye bağımlı olmak üzere iki şekilde bulunduğu gözlenmiştir. Ek olarak, bu çalışmada Bombyx mori testislerinden sürdürülebilir hücre hatları oluşturmak için yapışkan hücrelerin değil, süspanse hücrelerin daha uygun oldukları tespit edilmiştir

Anahtar Kelimeler:

Böcek hücre kültürü, larval testis, Bombyx mori, primer hücre kültürü

Primary Culture of Lepidopteran Adherent and Suspension Cells from Larval Testes of Bombyx mori

In this study, first aim is to establish primary insect cell culture from larval testes of Bombyx mori. The other aim this to investigate the appropriate cells for establishing continuous cell lines. Testes obtained from 5 instar larvae of B. mori were cultured in Grace’s insect medium. Consequently, it was seen that primary cell culture from testes of B. mori resulted in suspension and adherent cell lines. In addition, this study showed that while adherent cells were not appropriate choice to initiate continuous cell line, suspension cells were suitable to establish continuous cell lines from Bombyx mori testes.

Keywords:

Insect cell culture, larval testes, Bombyx mori, primary cell culture,

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