Zeynep ERBAŞ, İsmail DEMİR, Zihni DEMİRBAĞ

Avrupa Danaburnu Gryllotalpa gryllotalpa Orthoptera: Gryllotal- pidae ile İlişkili Parazitik Bir Nematod Olan Oscheius myriophila Nematoda: Rhabditida ’nın İzolasyonu ve Karakterizasyonu

B u çalışmada, Türkiye’de Karadeniz Bölgesi’nden toplanan Avrupa danaburnu Gryllotalpa gryllotalpa L., Orthoptera: Gryllotalpidae populasyonundan bir nematod suşu izole edildi. Suş, morfometrik ve moleküler ITS kısmi sekansı özelliklerine göre Oscheius myriophila olarak tanımlandı. Dauer jüvenillerin bu türleri Rhabditis myriophila Poinar, 1986 ’ya benzemekle birlikte, O. myriophila sahip olduğu; vücut uzunluğu 571.3693.9 ve baştan sinir halkasına olan uzunluk 100-116.8 , ve küçük kuyruk uzunluğu 53.4-76.8 ve anüs genişliği 10.4-13.8 özellikleriyle farklıdır. Bu evre, nematodu bir kılıf gibi kuşatan ikinci evre kütikül içinde bulunan üçüncü evre jüvenildir. rDNA’nın ITS bölgesinin sıraları bu tanımlamayı doğruladı. Bu tür G. gryllotalpa’da ilk kez kaydedildi

Anahtar Kelimeler:

Oscheius myriophila, Gryllotalpa gryllotalpa, parazitik nematod

Isolation and Characterization of a Parasitic Nematode, Oscheius myriophila Nematoda: Rhabditida , Associated with European Mole Cricket, Gryllotalpa gryllotalpa Orthoptera: Gryllotalpidae

Anematode strain was isolated from a population of European mole cricket, Gryllotalpa gryllotalpa L. Orthoptera: Gryllotalpidae , collected from the Black Sea Region of Turkey. Based on morphometrical and molecular ITS partial sequence properties, it was identified as Oscheius myriophila. This species of dauer juveniles resembled with Rhabditis myriophila Poinar, 1986 , however, it differs in having larger body length 571.3-693.9 and distance from the head to the nerve ring 100-116.8 , and smaller tail length 53.4-76.8 and width at anus 10.4-13.8 . This stage is the third-stage juvenile enclosed in a second-stage cuticle that surrounds the nematode like a sheath. The sequences of the ITS region of rDNA confirmed this identification. The species is recorded for the first time from G. gryllotalpa.

Keywords:

Oscheius myriophila, Gryllotalpa gryllotalpa, parasitic nematode,

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