Biyopsi Yönteminin Geliştirilen Üç Boyutlu Yapay Dokunun Kalitesine Olan Etkisi

B u çalışmanın amacı, biyopsi yönteminin oluşturulan yapay dokunun kalitesine olan etkisini incelemektir. Bu doğrultuda, lazer veya punch biyopsi yöntemleri kullanılarak elde edilen epitel ve fibroblast hücreleri ile iki tam kalınlıkta, üç boyutlu, yapay ağız mukozası modeli oluşturulmuştur. Sonrasında, bu modeller histoloji, immünohistokimya ve geçirimli elektron mikroskopisi kullanılarak incelenmişlerdir. Elde edilen sonuçlar, punch biyopsisi kullanılarak geliştirilen yapay ağız mukozasının morfolojik ve histolojik olarak doğalına çok benzediğini, fakat lazer biyopsisi kullanılarak elde edilen yapay mukozanın, doğal mukozadan farklı olarak istenmeyen bazı özellikler taşıdığını göstermiştir

Anahtar Kelimeler:

Doku mühendisliği, ağız mukozası, biyomalzemeler, biyopsi yöntemi

The Influence of The Biopsy Procedure on The Quality of a Three-Dimensional Reconstructed Artificial Tissue

The aim of the present study was to investigate the impact of the initial biopsy harvest procedure on the quality of the final reconstructed tissue. For this purpose, two full-thickness human oral mucosa models were reconstructed by tissue engineering from epithelial cells and fibroblasts obtained via punch or laser biopsy procedures and they were analyzed by using histology, immunohistochemistry, and transmission electron microscopy. The results showed that the oral mucosa model reconstructed by using punch biopsy mimicked very closely the native oral mucosa both morphologically and histologically; on the other hand, laser biopsy resulted in an artificial oral mucosa with less desirable properties.

Keywords:

tissue engineering, oral mucosa, biomaterials, biopsy method,

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