Batı Anadolu’da Yayılış Gösteren Meriones tristrami Rodentia: Gerbillinae Alttürlerinin Allozim Varyasyonları

Allozim varyasyonları, Batı Anadolu’daki Meriones tristrami’nin üç alt popülasyonunda 24 gen lokusunun elektroforetik analiziyle incelendi. Yirmidört lokusun yirmisi monomorfiktir ve altpopülasyonlar arasında aynı allelde fikse olmuştur, dört lokus ise polimorfiktir. Wright’ın fiksasyon indeksinin ortalama değeri FST = 0.0671, % 6.7’lik bir genetik varyasyon olduğunu göstermektedir ve bu da Meriones tristrami alttürlerinde biraz düşük bir farklılığın olduğuna işaret eder. Elde edilen FST değeri M. tristrami altpopülasyonları için ise orta derecede bir genetik farklılığı göstermektedir. Tüm altpopülasyonlar için ortalama heterozigotluk Ho= direk hesaplanan Ho= 0.017’dir, 0.01 ve 0.029 arasında değişir. Nei’nin genetik mesafesi düşüktür ve altpopülasyonlar arasında D = 0.000 - 0.002 arasında değişiklik göstermiştir. Göç sayısı Nm 3.48 değerinde bulunmuştur ve bu değer altpopülasyonlar arasında etkin gen akışının olduğunu da göstermiştir

Anahtar Kelimeler:

Allozim, alttür, Meriones tristrami, Türkiye

Allozyme Variations on Subspecies of Meriones tristrami Rodentia: Gerbillinae In Western Anatolia

Allozyme variation was investigated by the electrophoretic analysis of 24 gene loci in three subspecies of Meriones tristrami from Western Anatolia. Twenty of the twenty-four loci were monomorphic among populations, whereas four loci were polymorphic. The mean value of the Wright’s fixation index was FST = 0.0671, suggesting the existence of a slightly distinct subspecies in the Meriones tristrami populations. Overall mean heterozygosity Ho= direct count for all populations was Ho= 0.017. Nei’s measure of genetic distance was low and varied from D = 0.000 to 0.002 among populations. The number of migrants Nm equaled 3.48, which also suggests effective gene flow across populations..

Keywords:

Allozyme, subspecies, Meriones tristrami, Turkey,

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[1] O. Thomas, On two new Muridae from Smyrna, Ann. Mag. Nat. Hist., 9 (1903) 188.
[2] O. Thomas, Notes on Gerbils referred to the Genus Meriones with Descriptions of new species and subspecies, Ann. Mag. Nat. Hist., 9 (1919) 263.
[3] G. Neuhäuser, Die Muriden von Kleinasien, Z. Säugetierkd., 11 (1936) 161.
[4] R. Matthey, Cytologie et taxonomie du genre Meriones, IIIiger, Säugetierkd. Mitt., 5 (1957) 145.
[5] M. Baltazard, M. Bahmanyar, P. Mostachfi, M. Eftekhari, Ch. Mofidi, Recherches Sur la peste en Iran, Bull. World Health Org., 23 (1960) 141.
[6] D.L. Harrison, The Mammals of Arabia: Lagomorpha and rodentia, Vol. 3, Ernest Benn Ltd. London, England, 1972.
[7] D.L. Harrison, P.J.J. Bates, The Mammals of Arabia, Second edition, Harrison Zoological Museum Publication, Severoaks Kent, England, 1991.
[8] N. Yigit, E. Kıvanc, E. Colak, On the Taxonomic status of Meriones tristrami Thomas, 1892 (Rodentia: Gerbillinae) in Turkey, Zool. in the Middle East, 16 (1998a) 19.
[9] N. Yigit, E. Colak, A new subspecies of Meriones tristrami Thomas, 1892 (Rodentia: Gerbillinae) from Kilis (Southeastern Turkey); Meriones tristrami kilisensis subsp. n. Tr. J. of Zool., 22 (1998b) 99.
[10] R. Colak, N. Yigit, E. Colak, R. Gattermann, K. Neumann, SDS-PAGE Patterns of Blood Serum Proteins in some Species of the Genus Meriones (Mammalia: Rodentia), Tr. J. Zool., 26 (2002) 177.
[11] E. Nevo, M.G. Filippucci, C. Redi, S. Simson, G. Heth, A. Beiles, Karyotype and genetic evolution in speciation of subterranean mole rats of the genus Spalax in Turkey, Bio. Linn. Soc., 54 (1995) 203.
[12] M.G. Filippucci, G. Storch, M. Macholán, Taxonomy of the genus Sylvaemus in Western Anatolia morphological and electrophoretic evidence (Mammalia: Rodentia: Muridae). Senckenbergiana biol., 76 (1996) 1.
[13] M.G. Filipucci, B. Kryštufek, S. Simson, C. Kurtonur, B. Ozkan, Allozymic and biometric variation in Dryomys nitedula (Pallas, 1779). Proc. II Conf. on Dormice (Rodentia, Myoxidae), Hystrix, 6 (1994) 127.
[14] M. Macholán, M.G. Filippucci, J. Zima, Genetic variation and zoogeography of pine voles of the Microtus subterraneus / majori group in Europe and Asia Minor, J Zool., 255 (2001) 31.
[15] T. Kankılıc, Karyology and allozyme variations of Rattus (Fischer, 1803) (Rodentia; Muridae) species distributed in Turkish Thrace, PhD thesis (In Turkish), Ankara University, Graduate School of Natural and Applied Sciences, Besevler, Ankara, 2005.
[16] N. Yigit, T. Kankılıc, R. Colak, E. Colak, R. Gatterman, K. Neumann, Ş. Öztürk, M.G. Moradi, Allozyme Variations and Genetic Differentiation in Mesocricetus brandti Nehring 1898 and Mesocricetus auratus (Waterhouse, 1839) (Mammalia: Rodentia), Tr. J. Zool., 31 (2007) 227.
[17] R.C. Shaw, R. Prasad, Starch Gel Electrophoresis of Enzymes- A Compilation of Recipes, Biochemical Genetics, 4 (1970) 297.
[18] H. Harris, D.A. Hopkinson, Handbook of enzyme electrophoresis in human genetics, American Elsevier, New York, USA, 1976.
[19] W.C. Black, A Computer Program for the Analysis of Allelic Variation in Genetics, Modification of original version (BIOSYS-1) by David L. Swofford and Richard B. Selander, 1997.
[20] J.B.S. Haldane, A test for randomness of mating, J. Genet., 52 (1954) 631.
[21] H. Levene, On a matching problem arising in genetics, Ann. Math. Stat., 20 (1949) 91.
[22] S. Wright, The genetical structure of populations, Ann. Eugenics, 15 (1951) 323.
[23] S. Wright, The interpretation of population structure by F-statistics with special regard to systems of mating, Evolution, 19 (1965) 395.
[24] M. Nei, R.K. Chesser, Estimation of Fixation indices and gene diversities, Ann. Human Genet, 47 (1983) 253.
[25] M. Slatkin, N. Barton, A comparison of three indirect methods for estimating average level of gene flow, Evolution, 43 (1989) 1349.
[26] M. Nei, Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, 89 (1978) 583.
[27] R.R. Sokal, P.H.A. Sneath, Principles of numerical taxonomy, W.H. Freeman Co., San Francisco, 1963.
[28] F.J. Rohlf, NTSYS – pc, version 2.1. Numerical Taxonomy and Multivariate Analysis System, Exeter Publishing, LTD. New York, 2000.
[29] E. Nevo, Genetic variation in natural populations: patterns and theory, Theor. Pop. Biol, 13 (1978) 121.
[30] S. Wright, Evolution and the Genetics of Populations, Vol. 4, Variability Within and Among Natural Populations, University of Chicago, Chicago, 1978.
[31] E. Nevo, M.G. Filippucci, C. Redi, A. Korol, A. Beiles, Chromosomal speciation and adaptive radiation of mole rats in Asia Minor correlated with increased ecological stres, Proc. Natl. Acad. Sci. USA, 91 (1994) 8169.
[32] N. Yigit, K. Neumann, Ş. Ozturk, E. Colak, R. Colak, Biometric and Genetic Evaluation of Spermophilus (Mammalia: Rodentia) Populations in Western Turkey, Isr. J. Zool., 51 (2005) 191.