Meryem ÖZMAK, Ahu Gümrah DUMANLI, Yuda YÜRÜM, Neşe Burcu SAVAK, Abdülkerim KARABAKAN, Murat AKGÜL

Adsorption of bovine serum albumin (BSA) on clinoptilolite

The purpose of the present work was to investigate the adsorption of BSA on clinoptilolite in aqueous media. In this study, batch adsorption experiments were carried out and the effects of pH, protein concentration, ionic strength and modification of zeolite on the adsorption process were examined. The optimum pH for adsorption was found to be 4.0. It was found that various treatments applied to modify clinoptilolite has a substantial effect on the adsorption. In adsorption studies, residual BSA concentration reached equilibrium in a short duration of 60 min. Maximum adsorption capacity value for clinoptilolite in the original form is 388.3 mg BSA/g zeolite (23.2 mg BSA/m2) and in the modified form ranges from 500-660 mg BSA/g zeolite (25-33 mg BSA/m2) showed that this adsorbent, clinoptilolite, was suitable for the adsorption of BSA from aqueous media.

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1. Kondo, A., Oku, S., Higashitani, K., J. Colloid, Interface Sci.143 (1991) 214.
2. Haynes, C.A., Norde, W., Colloids Surf. B, Biointerfaces 2 (1994) 517.
3. Mizukami, F., Izutsu, H., T. Osaka, T., Akiyama, Y., Uiji, N., Moriya, K., Endo, K., Maeda, K., Kiyozumi, Y., Sakaguchi, K., J. Chromatogr, A 697 (1995) 279.
4. Akiyama, Y., Mizukami, F., Kiyozumi, Y. , Maeda, K., Izutsu, H., Sakaguchi, K., Angew. Chem. Int. Ed. 38 (1999)1420.
5. Kelleher, B.P., Doyle, A.M., O’Dwyer, T.F., Hodnett, B.K., J.Chem. Technol.Biotechnol, 76 (2001) 1216.
6. Breck, D.W., Zeolite Molecular Sieves, Wiley, London 1974.
7. Szostak, R., van Bekkum, H., Flanigen, E.M., Jansen, J.C., (Eds.), Modified Zeolites, Introduction to Zeolite Science and Practice, Studies in Surface Science and Catalysis, vol. 58, Elsevier, Amsterdam, 1991 (Chapter5).
8. Katiyar, A., Ji, L., Smirniotis, P.G., Pinto, N.G., Microporous and Mesoporous Materials, 80 (2005) 311.
9. Matsui, M., Kiyozumi, Y., Yamamoto, T., Mizushina, Y., Mizukami, F., Sakaguchi, K., Chem. Eur. J., 7 (2001) 1555.
10. Kondo, A., Oku, S., Murakami, F., Higashitani, K., Colloids Surf. B Biointerfaces, 1 (1993) 197.
11. Carter, D.C., and Ho, J.X., Adv. Protein Chem, 45(1994) 153.
12. Figge, J., Rossing, T.H., Fencl, V., J.Lab. Clin. Med, 117 (1991) 453.
13. Coradin, T., Coupe, A., Livage, J., Colloids and Surfaces B: Biointerfaces, 29 (2003) 189.
14. Tarasevich, Y.I., Theor. Exp. Chem, 37 (2001) 98.
15. Giacomelli, C.E., Norde,W., J. Colloid Interface Sci, 233 (2001) 234.
16. Buijs, J., Norde, W., Lichtenbelt, J.W.T., Langmuir, 12 (1996) 1605.
17. Tsu, J., Lu, J.R., Thomas, R.K., Cui, Z.F., Penfold, J., Langmuir, 14 (1998) 438.
18. Ji, L., Katiyar, A., Pinto, N.G., Jaroniec, M. and Smirniotis, P.G., Micropor. And Mesopor. Mater, 75 (2004) 221.
19. Norde, W., Favier, J.P., Colloids Surf, 64 (1992) 87.
20. Jeon, J.S., Raghavan, S., Sperline, R.P., Colloids Surf. A Physicochem. Eng. Aspects, 92 (1994) 255.
21. McClellan, SJ., Franses, E.I., Colloids Surf. A Physicochem. Eng. Aspects, 260 (2005) 265-8.
22. Chiku, H., Matsui, M., Murakami, S., Kiyozumi, Y., Mizukami, F., Sakaguchi, K., Analytical Biochemistry, 318 (2003) 80.
23. Cleland, J. L., Hedgepeth, C., Wang, D.L., J. Biol. Chem, 267 (1992) 13327.
24. Cleland, J. L., Builder, S. E., Swartz, J. R., Winkler, M., Chang, J. Y., Wang, D. I., Biotechnology (N.Y.), 10 (1992) 1013.
25. Molnar, A., Beregszaszi, T., Fudala, A., Lentz, P., Nagy, J.B., Konya, Z., Kiricsi, I., Catal. J., 202 (2001) 379.
26. He, J., Li, X., Evans, D.G., Duan, X., Li, C., J. Mol.Catal. B. Enzym, 11 (2000) 45.
27. Buijs, J., Hlady, V., J. Colloid Interface Sci, 190 (1997) 171.
28. Larsericsdotter, H., Oscarsson, S., Buijs, J., J.Colloid Interface Sci, 237 (2001) 98.
29. Vis, H., Heinemann, U., Dobson, C.M., Robinson, C.V., J. Am.Chem. Soc, 120 (1998) 6427.