Nurullah ŞANLI, Tuğrul YILDIRIM, Senem ŞANLI, Bediha AKMEŞE

A validated RP-HPLC method for the estimation of ornidazole in pharmaceutical dosage forms

Ornidazol için yeni, kolay, seçici, kesin ve doğru, izokratik ters faz sıvı kromatografik metot geliştirilmiş ve tablet formülasyonlarından miktar tayini için valide edilmiştir. Tinidazol iç standart olarak kullanılmıştır. Bu bileşikler için ayırma, X-Terra (Waters Corp., Milford, MA, USA) RP-18 kolonu ve mobil faz olarak 10 mM o-fosforik asit içeren pH 3’e ayarlanmış (30:70 v/v) asetonitril-su karışımı kullanılarak 1.0 mL dakika-1 akış hızında gerçekleştirilmiştir. Metot ICH kurallarına göre valide edilmiştir.

Ornidazol’ün farmasötik dozaj formlarından valide edilmiş RP-HPLC metodu ile belirlenmesi

A new, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid chromatography assay has been developed and validated for assay of Ornidazole in tablet formulations. Tinidazole was used as an internal standard. These compounds are separated well on a X-Terra (Waters Corp., Milford, MA, USA) RP-18 column by using a mobile phase consisting of a mixture of acetonitrile : water (30:70; v/v) adjusted to pH 3.00 with 10 mM o-phosphoric acid at a flow rate of 1.0 mL min-1. The method was validated as per the ICH guidelines.

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