Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads

Poly ethylene glycol dimethacrylate-n-vinyl imidazole [poly EGDMA-VIM ] hydrogel beads average diameter 150–200 µm was prepared by copolymerizing of ethylene glycol dimethacrylate EGDMA with n-vinyl imidazole VIM .Poly EGDMA-VIM beads had a specific surface area of 59.8 m2/g. Poly- EGDMA-VIM beads were characterized byswelling studies, elemental and SEM. Cu2+ions were chelated on the poly EGDMA-VIM beads, then these beadswere used in the reversible adsorption of mushroom tyrosinase. The maximum tyrosinase adsorption capacity of thepoly EGDMA-VIM –Cu2+beads was observed as 14.04 mg/g at pH 6.5. Storage stability was found to increase withimmobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss inadsorption capacity or enzyme activity

Keywords:

Enzyme immobilization, Tyrosinase Chelating beads, n-Vinyl imidazole, Affinity beads,

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S. Wada S. Ichikawa, K. Tatsumi, Biotechnol. Bioeng., 45 (1995) 304. 11. S. Davis, R.G. Burs, Appl Microbial Biotechnol., 32 (1990) 721.
R.S. Freire, N. Duran, L.T. Kubota, Talanta, 54 (2001) 681.
A.I. Yaropolov, A.N. Kharybin, J. Emneus, G. Marko- Varga, Anal Chim Acta, 308 (1995) 137.
A.V. Vakurov, A.K. Gladilin, A.V. Levashov, Y.L. Khmelnitsky, Biotechnol Lett., 16 (1994) 175.
C. Crecchio, P. Ruggiero, M.D.R. Pizzigallo, M. Curci, Dev Plant Soil Sci., 71 (1997) 545.
G. DeStefano, P. Piacquadio, V. Sciancalepore, Biotechnol. Technol., 10 (1996) 857.
A. Kara, L. Uzun, N. Besirli, A. Denizli, J. Hazard. Mater., 106 (2004) 93.
A. Kara, B. Osman, H. Yavuz, A. Denizli, React. Functl. Polym., 62 (2005) 61.
J. Rodakiewicz-Nowak, M. Monkiewicz, J. Haber, Colloids Surf. A 208 (2002) 347.
N. Duran, M.A. Rosa, A. D’Annibale, L. Gianfreda, Enzyme Microb.Technol., 31 (2002) 907.
A. Denizli, M. Alkan, B. Garipcan, S. Özkara, E. Pişkin, J. Chromatogr. B, 795 (2003) 93.
Y.H. Tsai, M.Y. Wang, S.Y. Suen, J. Chromatogr. A, 766 (2001) 133.
S. Emir, R. Say, H. Yavuz, A. Denizli, Biotechnol. Prog., 20 (2004) 223.