Eylem ÖZTÜRK GÜVEN, Murat DEMİRBİLEK, Necdet SAĞLAM, Zeynep KARAHALİLOĞLU, Ebru ERDAL, Cem BAYRAM, Emir Baki DENKBAŞ

Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications

Polyhydroxyalkanoates PHA are good alternatives on account of biocompatible and biodegradable properties toproduce materials as scaffolds for engineered tissues. Polyhydroxybutyrate PHB which is a member ofpolyhydroxyalkanoate family have been widely used as a biomaterial for in vitro and in vivo studies due to its uniqueproperties such as improved flexibility and processability. In this study polyhydroxybutyrate scaffolds were preparedfor tissue engineering applications. In order to improve cell attachment on the scaffolds they were modified. Duringthe modification three different immunologically inactive compounds, polyetyhylene glycol PEG , 2-hydroxyethylmethacrylate HEMA and ethylenediamine EDA were used in radio frequency glow discharge RFGD plasmapolymerization system. Morphological evaluations were obtained by using scanning electron microscopy. Obtainedresults showed high and interconnected porosity. In vitro weight loss profiles of the scaffolds were investigated byusing gravimetric method and found to be influenced by PHB concentration used in the preparation of scaffolds. Theirbiological promotion of activities including cell attachment, morphology and proliferation on L929 mouse fibroblast cellswere examined and cytotoxicity tests were performed at the last part of the study

Keywords:

Polyhydroxybutyrate, scaffold, tissue engineering, morphology, glow discharge,

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1. Yarlagadda, P.K., Chandrasekharan, M. and Shyan, J.Y.M., Recent advances and current developments in tissue scaffolding, BioMedical Materials and Engineering, 15 (2005) 159-177.
2. Williams, D., Benefit and risk in tissue engineering, Mater Today, 7 (2004) 24–29.
3. Mano, J.F., Sousa, R.A., Boesel, L.F., Neves, N.M., Reis, R.L, Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments, Compos. Sci. Technol., 64 (2004) 789–817.
4. Shin, H., Jo, S., Mikos, A.G., Biomimetic materials for tissue engineering, Biomaterials, 24 (2003) 4353–4364.
5. Drotleff, S., Lungwitz, U., Breunig, M., Dennis, A., Blunk, T., Tessmar, J., Biomimetic polymers in pharmaceutical and biomedical sciences, Eur. J. Pharm. Biopharm., 58 (2004) 385–407.
6. Suchanek, W., Yoshimura, M., Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants, J. Mater. Res., 13 (1998) 94–117.
7. McIntire, L.V. (Ed), WTEC Panel on Tissue Engineering Research: Final Report, Academic Press, San Diego, 2003.
8. Sachlos, E., Czernuszka, J.T., Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds, European Cells and Materials, 5 (2003) 29-40.
9. Whang, K., Healy, E., Elenz, D.R., Nam, E.K., Tsai, D.C., Thomas, C.H., Nuber, G.W., Glorieux, F.H., Travers, R., Sprague, S.M., Engineering bone regeneration with bioabsorbable scaffolds with novel microarchitecture, Tissue Eng. ,5 (1999) 35-51.
10. Chen, G.Q., and Wu, Q., The application of polyhydroxyalkanoates as tissue engineering materials, Biomaterials, 26 (2005) 6565–6578
11. Chen, L.J., Wang, M., Production and evaluation of biodegradable composites based on PHB–PHV copolymer, Biomaterials, 23 (2002) 2631–2639.
12. Hsu, Y.Y., Gresser, J.D., Trantolo, D.J., Lyons, C.M., Gangadharam, P.R.J., Wise, D.L. Effect of polymer foam morphology and density on kinetics of in vitro controlled release of isoniazid from compressed foam matrices, J Biomed. Mater. Sci., 35 (1997) 107-116.
13. Mansur, H.S., Costa, H.S., Nanostructured poly(vinyl alcohol)/bioactive glass and poly(vinyl alcohol)/chitosan/bioactive glass hybrid scaffolds for biomedical applications, Chemical Engineering Journal, 137 (2008) 72– 83.
14. Jiang, L., Li, Y., Wang, X., Zhang, L., Wen, J., Gong, M., Preparation and properties of nanohydroxyapatite / chitosan / carboxymethyl cellulose composite scaffold, Carbohydrate Polymers, 74 (2008) 680–684.
15. Wu, L., Ding, J., Effects of porosity and pore size on in vitro degradation of threedimensional porous poly(D,L-lactide-coglycolide) scaffolds for tissue engineering, Journal of Biomedical Materials Research, 75 (2005) 767-777.
16. Gong, S., Wang, H., Sun, Q., Xue, S., Wang, J., Mechanical properties and in vitro biocompatibility of porous zein scaffolds, Biomaterials, 27 (2006) 3793–3799.