Seçil UTKU, Erkut YILMAZ, Deniz TÜRKMEN, Lokman UZUN, Bora GARİPCAN, Rıdvan SAY, Adil DENİZLİ

Ion-Imprinted Thermosensitive Polymers for Fe3+ Removal from Human Plasma

Thermosensitive gels have attracted a great deal of attention for the applications of drug delivery systems, actuators,separation and removal of biological compounds and metals. Poly N-isopropylacrylamide poly NIPA , arepresentative gel, has a lower critical solution temperature LCST in the vicinity of 32°C i.e. showing hydrophilicityand hydrophobicity in water at lower and higher temperatures, respectively. Novel adsorbents using thermosensitivegels for trapping metals were reported where poly NIPA was used as a thermosensitive backbone polymer. Achelating group, which interacts with heavy metals, was introduced into poly NIPA with a molecular imprintingtechnique using a specific metal as the template. Although it is emphasized that metals play important roles inbiological processes and some of them are classified as essential, the toxic symptoms will manifest when a metal ionlevel exceeds a certain threshold level. Many symptoms of iron toxicity, for example heart attacks, diabetes, arthritis,depression and liver failure, are arised from the absorption of iron in unacceptably high concentrations because of agenetic failure or by accidental ingestion.The aim of this study is to prepare ion-imprinted polymersfor the removalof iron from aqueous solutions andsolutionsthalassemia patient`s plasma.N-methacryloyl- L -cysteine MAC wasselected as the metal complexing monomer, with the goal preparing a solid-phase which has a high selectivity for Fe3+ions. Poly NIPA was used as a thermosensitive backbone polymer matrix. A chelating group [N-methacryloyl- L cysteine MAC ] which interacts with Fe3+ions, was introduced into poly NIPA with a molecular imprinting technique.After removal of the Fe3+ions, adsorption of Fe3+ions from thalassemia patient’s plasma both on the poly NIPA-MAC and Fe3+-imprinted poly NIPA-MAC particles were studied in batch-wise

Keywords:

thermosensitive polymers, poly NIPA, Ion imprinting, Thalassemia, Affinity binding,

PDF

___

Tokuyama H., Fujioka M. and Sakohara S., 2005, J. Chem. Eng. Jpn. 38, p. 633.
Tokuyama H., Kanazawa R. and Sakohara S., 2005, Sep. Purif. Technol. 44, p. 152.
Wulff, G., 1998, Chem. Tech. 28, p. 19.
Garcia, R., Pinel, C., Madic, C., Lemaire, M., 1998, gadolinium/lanthanum separation, Tetrahedron Lett., 39, 8651–8654. effect in
Wulff, G., 1995, Molecular imprinting in cross- linked materials with the aid of molecular templates-a way towards artificial antibodies, Angew. Chem. Int. Ed. Engl., 34, 1812–1832.
Nishide, H., Tsuchida, E., 1976, Makromol. Chem., 177, 2295.
Kabanov, V.A., Efendiev, A.A., Orujev, D.D., 1979, Complex-forming polymeric sorbents with macromolecular arrangement favorable for ion sorption, J. Appl. Polym. Sci., 24, 259– 267.
Tsukagoshi, K., Kai, Y.Y., Maeda, M., Takagi, M., 1993, Bull. Chem. Soc. Jpn., 66, 114.
Dai S., Burleig M.C., Shin Y., Morrow C.C., Barnes C.E., Xue Z., 1999, Imprint coating: a novel synthesis of selective functionalized ordered mesoporous sorbents., Angew. Chem. Int. Ed., 38 (9) 1235–1239.
S., Burleig M.C., Ju Y.H., Gao H.J., Lin J.S., Pennycook S.J., Barnes C.E., Xue Z.L., 2000, Hierarchically imprinted sorbents for the separation of metal ions., J. Am. Chem. Soc., 122 (5) 992–993.
Andac¸ M., Say, R., Denizli, A., 2004, Molecular recognition based cadmium removal from human plasma, J. Chromatogr. B, 811, 119– 126.
Andaç, M., Mirel, S., Senel, S., Say, R., Ersoz, A., Denizli, A., 2007, Ion-imprinted beads for molecular recognition based mercury removal from human serum, Int. J. Biol. Macromol. 40, 159–166.
Weatherall, D.J., and Clegg, J.B.,1996, Thalassemia-a global public health problem. Nature. Med. 2 (1996), pp. 847–849.
Rund, D., Rachmilewitz, E.A., 1995, Advances in the pathophysiology and treatment of thalassemia. Critical Reviews in Hematology- Oncology, 20, 237-254.
Rund, D., Rachmilewitz, E.A., 2000, New trends in the treatment of beta-thalassemia. Critical Reviews in Oncology/Hematology, 33, 105–118.
Doumas B. R., Watson W., Biggs H., 1971, Clin. Chim. Acta, 31, 87.
Lagergren M., Ollsson P., Sweedenborg J., 1974, Surgery, p. 643.
Brash, J.L., 1991, Modern Aspects of Protein Adsorption on Biomaterials, Missirlis, Y.F., Lemm, W.Eds., Kluwer Academic Publishers, 39-47.
Wu, X.S., Hoffman, A.S., and Yager, P., 1992, Synthesis and characterization of thermally reversible macroporous poly(N-isopropyl acrylamide) hydrogels. J. Polym. Sci. Polym. Chem. 30, 2121–2129.
Zhang, X.Z., and Zhuo, R.X., 1999, Preparation of fast responsive, temperature sensitive hydrogel. Macromol. Chem. Phys., 200, 2602- 2605. amide)
Zhang, X.Z., Yang, Y.Y., Chung, T.S., and Ma, K.X., 2001, Preparation and characterisation of the macroporous poly(N-isopropylacrylamide) hydrogel with fast response, Langmuir 17, 6094–6099.
Schild H.G., Poly(N-isopropylacrylamide): experiment, theory and application, 1992, Prog. Polym. Sci., 17, 163–249.
Zhang, X.Z., Wu, D.Q., and Chu, C.C., 2003, Effect of the crosslinking level on the properties of temperature-sensitive poly(N-isopropyl acrylamide) hydrogels, Journal of Polymer Science Part A: Polymer Physics, 41, 582–593.
Lezzi, A., Sandra, S., and Roggero, A., 1994, Synthesis of thiol chelating resins and their adsorption properties toward heavy metal ions. J. Polym. Sci. Part A: Polym. Chem. 32, 1833– 1877.
Cooper, S.L., Bamford, C.H., Tsutura, T., (Ed) 1995, Polymer biomaterials in solution as interfaces and as Solids, VSP, Ultrecht, The Nederlands.
Pişkin, E., Hoffman, A., 1986, Polymeric Biomaterials, Dordrecht, The Netherlands, Martinus Nijhoff Pupl. Co.
Denizli, A., 1999, J. App. Polym. Sci., 74, 655- 662.
Kim, S.W., Jacops, H.,1996, Blood Purification, 14, 357-372.