Halil Sezgin SEMİS, Sinan ÇELİK, Ahmet KEKEÇ

Karpal Tünel Sendromu Cerrahisi Uygulanan Hastalarda Pnömotik Turnikenin Serum TAS, TOS, MDA ve GSH Seviyeleri Üzerine Etkileri

Bu çalışma karpal tünel sendromu cerrahisi uygulanan 25 hastada pnömotik turnike uygulamasının serum total oksidatif stres (TOS), total antioksidan kapasite (TAS), malondialdehit (MDA) ve glutatyon (GSH) düzeylerinde turnikenin farklı zamanlarında meydana gelen değişimlerin ortaya konulmasını amaçlamak için tasarlanmıştır. Çalışmaya karpal tünel sendromu tanısı alan ve cerrahi müdahale yapılan 25 hasta dahil edilmiş olup, hastalarının tamamında pnömotik turnike uygulandı. Hastalardan turnike uygulamasından 10 dakika önce, turnike uygulandıktan sonra 20. dakikada ve turnike açıldıktan 10 dakika sonra alınan kanlar 3500 rpm’de santrifüj edilerek serumları çıkarıldı. Elde edilen serumlardan TOS, TAS, MDA ve GSH düzeyleri kolorimetrik metodla spektrofotometrede bakıldı. Hastalardan elde edilen sonuçlar, turnike uygulamalarından sonra MDA ve TOS seviyelerinin yükseldiği, GSH ve TAS seviyelerinin azaldığını göstermiştir. Sonuç olarak, günümüzde sıklıkla görülen ve teşhisi konulmuş hastalara yaygın olarak yapılan karpal tünel sendromu ameliyatlarında cerrahi konforu artırmak amacıyla başvurulan turnike uygulamasına bağlı gelişen iskeminin ve turnike açıldıktan sonra gelişecek olan reperfüzyonun serum TOS, TAS, MDA ve GSH düzeyleri üzerinde nasıl bir değişim ortaya çıkardığı bu çalışma ile belirlenmiş olup, yapılacak olan çalışmalara katkı sunacaktır.

Anahtar Kelimeler:

Glutatyon, Karpal tünel sendromu, Malondialdehit, Total antioksidan kapasite, Total oksidatif stres

The Effects of Pneumatic Tourniquet on Serum TAS, TOS, MDA and GSH Levels in Patients Undergoing Carpal Tunnel Syndrome Surgery

This study was designed to reveal the changes in serum total oxidative stress (TOS), total antioxidant status (TAS), malondialdehyde (MDA) and glutathione (GSH) levels of pneumatic tourniquet application at different times of tourniquet in 25 patients who underwent carpal tunnel syndrome surgery. Twenty-five patients who were diagnosed with carpal tunnel syndrome and underwent surgical intervention were included in the study, and pneumatic tourniquets were applied to all patients. The blood taken from the patients 10 minutes before the tourniquet application, 20 minutes after the tourniquet application and 10 minutes after the tourniquet was opened, was centrifuged at 3500 rpm and the serum was removed. TOS, TAS, MDA and GSH levels of serums obtained were measured in a spectrophotometer using the colorimetric method. The results obtained from the patients showed that MDA and TOS levels increased and GSH and TAS levels decreased after tourniquet applications. As a result, how the ischemia due to tourniquet application, which is applied to increase surgical comfort, and reperfusion that will develop after the tourniquet is opened, on serum TOS, TAS, MDA and GSH levels in carpal tunnel surgery syndrome surgeries, which are frequently seen and commonly performed on patients with diagnosed today, change was determined by this study.

Keywords:

Glutathione, Carpal tunnel syndrome, Malondialdehyde, Total antioxidant status, Total oxidative stress,

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