Azer ÖZAD DÜZGÜN, Esma AKYILDIZ, Ayşegül SARAL, Tuba KÖSE

Klinik E.coli İzolatlarında Virulans Faktor Genlerinin ve Geniş Spekturumlu Beta Laktamazların Araştırılması

Bu çalışmanın amacı, 83 klinik E.coli izolatında pap, hly, cnf, sfa,aer ve afa virulans genlerinin ve geniş spekturumlu beta laktamaz direnç genlerinin varlığını araştırmaktır. 83 E. coli izolatında sfa, pap, hly, cnf, aerve afa virülans genleri ve TEM, SHV, CTX-M1 ve CTX-M9 geniş spekturumlu beta laktamaz genleri polimer zincir reaksiyonu (PZR) metodu ile araştırılmıştır. 83 izolatın, sefiksim, trimethoprim/sulfametaksazol, piperasilin/tazobaktam, amoksisilin/klavulanik asit, ampisilin, seftriakson, sefuroksim, sefuroksim aksetil, siprofloksasin, fosfomisin, gentamisin ve seftazidime karşı direnç oranları sırasıyla;%65, %54, %19, %38, %74, %75, %72, %75, %68, %2, %30, %49 olarak belirlenmiştir. CTX-M1, araştırılan genişlemiş spektrumlu beta-laktamaz (GSBL) genleri arasında 83 suşun 51’inde belirlenmesiyle en sık görülen GSBL olmuştur. TEM tipi beta laktamaz 33 suşta, CTX-M9 ve SHV 1 izolatta tespit edilmiştir. İzolatlarda en yaygın virülans geni, 40 suşta tespit edilmesiyle aer geni, bunu izolatların 15'inde tespit edilen cnf geni takip etmiştir. Sfa, pap, afavehlyvirulans genleri sırasıyla izolatların %15.6'sında, %15.6'sında, % 9.6'sında ve %12.04’ünde görülmüştür. Ayrıca 83 E.coliizolatında on farklı virulans faktör gen paterni tespit edilmiştir. Virulans gen kombinasyonları, 27 suştaaer, 6 suştacnf-sfa-pap, 1 suştaaer-pap, 2 suştaaer-afa, 6 suştaafa, 3 suştasfa, 9 suştaaer-cnf-hly, 4 suştasfa-pap, 1 suştahly-papve 1 suştapap olarak gözlemlenmiştir. Sonuç olarak, İYE ile ilişkili bakteriyel patojenitenin araştırılması daha iyi bir tıbbi müdahaleye katkı sağlayacaktır.

Anahtar Kelimeler:

Direnç, E.coli, Virulans

Investigation of Virulence Factor Genes and Extended Spekturum Beta Lactamases in Clinical E.coli Isolates

The aim of this study was to investigate the presence of pap, hly, cnf, sfa, aer and afa virulans genes and extended-spectrum beta lactamase resistance genes in 83 clinical E.coli isolates. In 83 E. coli isolates, sfa, pap, hly, cnf, aer and afa virulence genes and TEM-SHV, CTX-M1 and CTX-M9 wide spectrum beta-lactamase genes were investigated by polymerase chain reaction (PCR) method. Resistance rates of 83 isolates to cefixim, trimethoprim/sulfamethoxazole, piperacillin/tazobactam, amoxicillin/clavulanic acid, ampicillin, ceftriaxone, cefuroxime, cefuroxime axetil, ciprofloxacin, fosfomycin, gentamicin and ceftazidime antibiotics were 65%, 54%, 19%,38, 74%, 75%, 72%, 75%, 68%, 2%, 30%, 49%, respectively. CTX-M1 was the most common extended spectrum beta-lactamase (ESBL) among the ESBL genes investigated in 51 of 83 strains. TEM type beta-lactamase was detected in 33 strains, CTX-M9 and SHV 1 isolates. The most common virulence gene was aer gene and detected in 40 strains, followed by the cnf gene detected in 15 of the isolates. The pap, hly, afa and sfa genes were determined in 15.6%, 12.04%, 9.6% and 15.6% of the isolates, respectively. In addition, 10 different virulence factor gene patterns were observed in 83 E.coli isolates. Virulence gene combinations were observed in 27 strains of aer, 6 strains of cnf-sfa-pap, 1 strains of aer-pap, 2 strains of aer-afa, 6 strains of afa, 3 strain of sfa, 9 strain of aer-cnf-hly, 4 isalates of sfa-pap, 1 strain of hly-pap and 1 strains of pap genes. In conclusion, investigating bacterial pathogenicity associated with UTI will contribute to better medical intervention.

Keywords:

Resistance, E.coli, Virulence,

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