Ayşegül SARAL, Esma AKYILDIZ, Tuba KÖSE, Azer ÖZAD DÜZGÜN

Klinik E.coli İzolatlarında Virülans Faktör Genlerinin ve Genişlemiş Spektrumlu Beta Laktamazların Araştırılması

Bu çalışmanın amacı, 83 klinik E.coli izolatında pap, hly, cnf, sfa, aer ve afa virulans genlerinin ve genişlemişspektrumlu beta laktamaz direnç genlerinin varlığını araştırmaktır. Antibiyotik duyarlılık testi için 17 farklı antibiyotikkullanılmıştır. 83 E. coli izolatında sfa, pap, hly, cnf, aer ve afa virülans genleri ve TEM, SHV, CTX-M1 ve CTX-M9genişlemiş spektrumlu beta laktamaz genleri polimer zincir reaksiyon (PZR) metodu ile araştırılmıştır. 83 izolatın,sefiksim, trimethoprim/sulfametaksazol, piperasilin/tazobaktam, amoksisilin/klavulanik asit, ampisilin, seftriakson,sefuroksim, sefuroksim aksetil, siprofloksasin, fosfomisin, gentamisin ve seftazidime karşı direnç oranları sırasıyla;%65, %54, %19, %38, %74, %75, %72, %75, %68, %2, %30, %49 olarak belirlenmiştir. CTX-M1, araştırılangenişlemiş spektrumlu beta-laktamaz (GSBL) genleri arasında 83 suşun 51’inde belirlenmesiyle en sık görülen GSBLolmuştur. TEM tipi beta laktamaz 33 suşta, CTX-M9 ve SHV 1 izolatta tespit edilmiştir. İzolatlarda en yaygın virülansgeni, 40 suşta tespit edilmesiyle aer geni, bunu izolatların 15’inde tespit edilen cnf geni takip etmiştir. Sfa, pap, afa vehly virulans genleri sırasıyla izolatların %15.6’sında, %15.6’sında, %9.6’sında ve %12.04’ünde görülmüştür. Ayrıca 83E.coli izolatında on farklı virulans faktör gen paterni tespit edilmiştir. Virulans gen kombinasyonları, 27 suşta aer, 6suşta cnf-sfa-pap, 1 suşta aer-pap, 2 suşta aer-afa, 6 suşta afa, 3 suşta sfa, 9 suşta aer-cnf-hly, 4 suşta sfa-pap, 1 suştahly-pap ve 1 suşta pap olarak gözlemlenmiştir. Sonuç olarak, İYE ile ilişkili bakteriyel patojenitenin araştırılması dahaiyi bir tıbbi müdahaleye katkı sağlayacaktır.

Investigation of Virulence Factor Genes and Extended Spectrum Beta Lactamases in Clinical E.coli Isolates

The aim of this study was to investigate the presence of pap, hly, cnf, sfa, aer and afa virulence factor genes and extended-spectrum beta lactamase resistance genes in 83 clinical E.coli isolates. 17 different antibiotics were used for antibiotic susceptibility testing. In 83 E. coli isolates, sfa, pap, hly, cnf, aer and afa virulence genes and TEM-SHV, CTX-M1 and CTX-M9 extended spectrum beta-lactamase genes were investigated by polymerase chain reaction (PCR) method. Resistance rates of 83 isolates to cefixim, trimethoprim/sulfamethoxazole, piperacillin/tazobactam, amoxicillin/clavulanic acid, ampicillin, ceftriaxone, cefuroxime, cefuroxime axetil, ciprofloxacin, fosfomycin, gentamicin and ceftazidime antibiotics were 65%, 54%, 19%, 38%, 74%, 75%, 72%, 75%, 68%, 2%, 30%, 49%, respectively. CTX-M1 was the most common extended spectrum beta-lactamase (ESBL) among the ESBL genes investigated in 51 of 83 strains. TEM type beta-lactamase was detected in 33 strains, CTX-M9 and SHV in 1 isolates. The most common virulence gene was aer gene and detected in 40 strains, followed by the cnf gene detected in 15 of the isolates. The pap, hly, afa and sfa genes were determined in 15.6%, 12.04%, 9.6% and 15.6% of the isolates, respectively. In addition, 10 different virulence factor gene patterns were observed in 83 E.coli isolates. Virulence gene combinations were observed as aer (27/83), cnf-sfa-pap (6/83), aer-pap (1/83), aer-afa (2/83), afa (6/83), sfa (3/83), aer-cnf-hly (9/83), sfa-pap (4/83), hly-pap (1/83) and pap (1/83). In conclusion, investigating bacterial pathogenicity associated with UTI will contribute to better medical intervention.

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