Ardahan ve Çevresinde Yabani Olarak Yetişen Gilaburu Meyve Ekstraktının Antimikrobiyal, Antioksidan ve Antimutajenik Aktivitelerinin Araştırılması

Bu çalışmada Ardahan ve çevresinde doğal olarak yetişmekte olan Gilaburu meyvelerinin antioksidan özellikleri, farklı mikroorganizmalar üzerindeki antimikrobiyal etkisi ve Ames testi ile antimutajenik etkisi araştırıldı. Antimikrobiyal aktivite testleri esnasında farklı Gr (+) ve Gr (-) bakteriler ile mayalar kullanıldı. Antimutajenik etki çalışmalarında ise AMES/Salmonella Mikrozom testi ile Salmonella typhimurium TA 98 ve TA 100 suşları kullanıldı. Elde edilen verilere göre gilaburu meyvelerinin ağırlığı ortalama olarak 0.80 g, suda çözünür kuru madde (SÇKM) içeriği % 11.3, pH’sı 3.20, toplam antosiyanin miktarı 0.80 mg/g,1,1-diphenyl-2-picrylhydrazyl (DPPH)radikal süpürücü etkisi % 52.5, 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) değeri 72.8 µmol TE/g FW, Ferric Reducing Antioxidant Power (FRAP) değeri ise 122.6 µmol Fe II/g FW olarak belirlendi. Ayrıca gilaburu ekstraktının test mikroorganizmaları üzerinde değişen oranlarda antibakteriyel aktiviteye sahip oldukları; ancak antifungal aktivite sergilemedikleri belirlendi. Test bakterileri içerisinde ise en duyarlı olanının Pseudomonas aeruginosa ATCC 9027 olduğu belirlendi. Antimutajenite deneylerinde,TA98 suşlarında gilaburunun sadece 40 µl dozunda pozitif kontrole göre önemli bir azalma meydana getirdi. TA100 suşlarında ise hiçbir dozda pozitif kontrole göre istatistiksel olarak önemli bir azalma meydana gelmediği belirlendi.

Anahtar Kelimeler:

Antimikrobiyal, Antimutajen, Antioksidan, Gilaburu, Viburnum opulus

Investigation of Antimicrobial, Antioxidant, and Antimutagenic Activities of Wild Guelder RoseFruit Extract Grown in Ardahan and Its Surrounding Area

In this study, antioxidant activity, antibacterial effect against some test bacteria, and antimutagenic effect with AMES test of Guelder rose fruit naturally growing in Ardahan and its surrounding area were examined. Different Gr (+), and Gr (-) bacteria and yeasts were used as test microorganisms during the antibacterial activity tests. AMES / Salmonella/Microsome test was preferred for antimutagenic effect study and Salmonella typhimurium TA 98 and TA 100 strains were used. According to the obtained data, 0.80 g fruitweight on average, 11.3% of Soluble Dry Matter content, 3.20 of pH, 0.80 mg/g of total anthocyanin, and 52.5% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 72.8 µmol TE/g FW 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) value, 122.6 µmol Fe II/g FW Ferric Reducing Antioxidant Power (FRAP) value were determined. It was determined that Guelder rose fruit extract has antibacterial activity at different rates on test bacteria, but not antifungal activity. The most sensitive bacterium was Pseudomonas aeroginosa ATCC 9027 among the test microorganisms. In antimutagenic activity, only 40 μl dose of Guelder rose in TA98 strains caused a significant reduction compared to the positive control. In TA100 strains, no statistically significant decrease compared to positive control at any dose occurred.

Keywords:

Antimicrobial, Antimutagenic, Antioxidant, Guelder rose, Viburnum opulus,

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