In this study, 15 samples, recovered from depth were examined and evaluated for the ostracoda assemblage in the region of Gökçeada, Bozcaada and Çanakkale (Northeastern Aegean Sea). Ostracodes of Holocene (Late Quaternary) belong to 15 family, which consist of 37 genera and 61 species . Observed fauna was compared to the previous investigations, carried out within the various parts of Aegean Sea, Marmara Sea, Mediterranean and Atlantic Ocean. Described fauna in this study area, such as Cytherella vandenboldi Sissingh, C. vulgata Ruggieri, Callistocythere littoralis (Miiller), C. intricatoides (Ruggieri), C. pallida (Miiller), C. montana Doruk, Cyprideis torosa (Jones), Pterygocythereis ceratoptera (Bosquet), P. jonesii (Baird), Buntonia sublatissima sublatissima (Neviani), Urocythereis margaritifera (Mueller), U. sororcula (Sequenza), Caudites calceolatus (Costa), Bosquetina carinella (Reuss), B. dentata (Miiller), Semicytherura acuminata (Mueller), S. acuticostata (Sars), S. dispar (Mueller), S. inversa (Sequenza), indicates that close relation to the fauna observed in the region of Aegean Sea and Marmara Sea. Another ostracod species, such as Cushmanidea elongata (Brady), Neocytherideis cylindrica (Brady), Olimfalunia quadridentata (Baird), Loxoconcha turbida Miiller, L. granulata Sars, Paracytheridea depressa (Mueller), Eucytherura gibbera Mueller, Xestoleberis aurantia (Baird), Aglaiocypris complanata Brady & Robertson were similar with the fauna described in the region of Mediterranean and Atlantic Ocean.Ostracod species of Cytheridea acuminata neapolitana Kollmann, C. acuminata acuminata Bosquet, Acanthocythereis hystrix (Reuss), Carinocythereis carinata (Roemer), C. antiquata (Baird), Urocythereis margaritifera (Mueller), Cyclocypris globosa (Sars) were widely observed in the region of Mediterranean, Aegean Sea and Marmara Sea. The fauna of the study was generally observed in muddy and sandy samples between Gökçeada and Bozcaada, and at the beginning and final locations of the Bosphorus Dardanelles. In general, ostracodes have epineritic-infraneritic and bathyal zone features. Otherwise, some samples, including marine ostracodes as well as brackish and littoral specimens, represent fresh water derived from continents and deep sea flour fresh water springs in the near field.