Ercan ÖZDEMİR, Ayşe DEMİRKAZIK, Ahmet Şevki TAŞKIRAN, Olca KILINÇ, Gökhan ARSLAN

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Nitrik Oksit Sentaz NOS İnhibitörlerinin Sıçanlarda Çok Düşük Frekanslı Manyetik Alanın İndüklediği Analjezi Üzerine Etkileri

Amaç: Elektromanyetik alanın EMA farklı ağrı türlerini azalttığı bilinmektedir. Bununla birlikte, manyetik alanın analjezik etki mekanizması tam olarak anlaşılamamıştır. Bu çalışmanın amacı, nitrik oksit sentaz NOS inhibitörlerinin sıçanlarda çok düşük frekanslı EMA maruziyeti ile oluşan analjezi üzerine etkilerini araştırmaktır.Gereç ve Yöntem: Bu çalışmada 72 yetişkin erkek Wistar albino sıçan yaklaşık 230 ± 12 g ağırlığında kullanıldı. Sıçanlar, 22 ± 2 °C oda sıcaklığında, 12 saat aydınlık/karanlık siklusun sağlandığı ve ses yalıtımı olan ortamda tutuldu. Elektromanyetik alan 50 Hz , her gün dört defa 30 dakika süre ve 15 dakika aralıklar ile 15 gün boyunca uygulandı. Analjezik etki ölçümü tail-flick ve hot-plate testleri ile gerçekleştirildi. Analjezi testinden önce sıçanlara nitrik oksit donörü SNAP 30 mg/kg ve NOS inhibitörleri L-NAME 40 mg/kg ve 7-NI 25 mg/kg intraperitoneal olarak enjekte edildi. Verilerin istatistiksel analizinde varyans analizi iki yönlü ANOVA kullanılmış ve çoklu karşılaştırma Tukey testleri ile yapıldı. İstatistiksel olarak anlamlılık düzeyi p
Anahtar Kelimeler:

Analjezi, Elektromanyetik alan, NOS inhibitörleri, L-NAME, 7-NI

Objective: It is known that the electromagnetic field EMF reduces the different types of pain. However, the mechanism of magnetic field analgesia is not fully understood. Our aim of this study is to investigate the effects of nitric oxide synthase NOS inhibitors on analgesia induced by extremely low frequency EMF in rats.Material and Methods: In this study were used 72 adult male Wistar albino rats approximately 230 ± 12 g . The rats were provided environment where is at 22 ± 2 °C room temperature, 12-hour light/dark cycle and insulated from sound. The application of electromagnetic field 50 Hz , the same times for 30 minutes each day for 15 days, and a total of four times every 15 minute intervals. The analgesic effect measurement was performed by tail-flick and hot-plate tests. Prior to analgesia test, nitric oxide donor SNAP 30 mg/kg and NOS inhibitors L-NAME 40 mg/kg and 7-NI 25 mg/kg were injected intraperitoneally in rats. In the statistical analyzes of the data, analysis of variance two-way ANOVA was used and the multiple comparison determined by Tukey tests. The level of statistically significant was expressed p
Keywords:

Analgesia, Electromagnetic field, NOS inhibitors, L-NAME, 7-NI,

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Genel Tıp Dergisi-Cover
  • ISSN: 2602-3741
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1997
  • Yayıncı: SELÇUK ÜNİVERSİTESİ > TIP FAKÜLTESİ
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Nitrik Oksit Sentaz NOS İnhibitörlerinin Sıçanlarda Çok Düşük Frekanslı Manyetik Alanın İndüklediği Analjezi Üzerine Etkileri

Ercan ÖZDEMİR, Ayşe DEMİRKAZIK, Ahmet Şevki TAŞKIRAN, Olca KILINÇ, Gökhan ARSLAN