Fazile Cantürk TAN, Arzu Hanım YAY, Güler TOPRAK, Oğuz Galip YILDIZ

Gama Radyasyonun İndüklediği Nefrotoksiteye Karşılık Pycnogenolün Koruyucu Etkisi

Amaç: Gama radyasyonun indüklediği nefrotoksiteye karşı pycnogenolün radyasyon koruyucu ve antioksidan etkisi tek hücre düzeyinde, doku düzeyinde ve biyokimyasal parametreler kullanarak Wistar Albino cinsi erişkin erkek sıçanda araştırıldı. Gereç ve Yöntem: Çalışma, kontrol grubu n=10 , antioksidan gruplar P37.5, P75, P150, P300, n= 10 , sadece radyasyon grubu y-ışını, n=10 ve antioksidan + radyasyon gruplar P37.5+y-ışını, P75+y-ışını, P150+y-ışını, P300+y-ışını, n= 10 olmak üzere 100 sıçanda yapıldı. Pycnogenol %5’lik DMSO’da çözdürüldü. Radyasyon Cobalt-60 teleterapi cihazıyla tek fraksiyon uygulandı. Kontrol ve radyasyon grubuna %5 DMSO, antioksidan ve antioksidan + radyasyon gruplar ışınlamadan önce 24, 48 ve 72. saatlerde pycnogenol verildi. Uygulamalardan yirmidört saat sonra, genel anesteziyle sıçanlar sakrifiye edilerek böbrekleri alındı. Böbrek dokusunda, DNA hasarı, histo-patolojik değerlendirme, CAT, SOD aktiviteleri ve MDA düzeyleri değerlendirildi. Bulgular: Radyasyon grubunda MDA düzeyinde artma, SOD ve CAT aktivitelerinde azalma; buna karşılık antioksidan + radyasyon grubunda MDA düzeyinde azalma, SOD ve CAT aktivitelerinde artma tespit edildi. Kontrole göre diğer gruplarda comet parametreleri artmış, bu parametreler antioksidan + radyasyon gruplarda radyasyon grubuna göre azalmıştı p

Anahtar Kelimeler:

Böbrek, radyasyon, antioksidan, pycnogenol

Protective effect of pycnogenol against gamma radiation-induced nephrotoxicity

Objective: The radioprotective and antioxidant effect of pycnogenol against gamma radiation-induced nephrotoxicity was investigated at single cell level, tissue level and biochemical parameters in adult male Wistar Albino rats.Material and Methods: The study was included control group n=10 , antioxidant groups P37.5, P75, P150, P300, n= 10 , only radiation group y-ışını, n=10 and antioxidant + radiation groups P37.5+y-ışını, P75+y-ışını, P150+y-ışını, P300+y-ışını, n= 10 on 100 rats. Pycnogenol was dissolved in 5 % DMSO. Radiation was applied single fraction with Cobalt-60 teletherapy device. Control and radiation groups were received 5% DMSO. Antioxidant and antioxidant + radiation groups were received pycnogenol at 24, 48 and 72 hours before the irradiation. Twenty-four hours after the administration, the rats were sacrificed with general anesthesia and their kidneys were removed. DNA damage, histopathological evaluation, CAT, SOD activities and MDA levels were evaluated in renal tissue. Results: We were evaluated increased levels of MDA, decreased SOD and CAT activities in the radiation group; however, MDA level was decreased and increased SOD and CAT activities in the antioxidant + radiation group. Comet parameters were increased in the other groups compared to the control group and these parameters were decreased in the antioxidant + radiation groups compared to the radiation group p

Keywords:

Kidney, radiation, antioxidant, pycnogenol,

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ISSN: 2602-3741
Yayın Aralığı: Yılda 6 Sayı
Başlangıç: 1997
Yayıncı: SELÇUK ÜNİVERSİTESİ > TIP FAKÜLTESİ

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