Apigenin-7-Glikozit Antioksidan Enzim Sisteminin Aktivasyonu ve Kaspaz Genlerinin Ekspresyonunun İnhibisyonu Yoluyla SH-SY5Y Hücrelerinde Hidrojen Peroksit İndüklü Oksidatif Stresi ve Nöronal Ölümü Azaltır

ÖZ Amaç: Bu çalışmada antikanser ve anti-inflamatuar etkinliği bilinen bir doğal ürün olan Apigenin-7-Glikozitin (AP7Glu), SH-SY5Y hücrelerinde hidrojen proksit (H2O2)-indüklü oksidatif strese karşı olası nöroprotektif etkisinin araştırılması amaçlandı. Yöntem: H2O2 ve AP7Glu’nun hücre canlılığı üzerine etkisi XTT analizi ile belirlendi. Antioksidan enzim aktivitelerinin belirlenmesine yönelik olarak SOD ve katalaz analizleri gerçekleştirildi. Antioksidan savunma sistemi ile ilişkili genler ile Kaspaz genlerinin ekspresyon seviyeleri qRT-PCR analizi ile değerlendirildi. Bulgular: SH-SY5Y hücrelerine 24 saat süresince 300 µM H2O2 uygulaması sonrasında hücre canlılığı % 63.18 olarak belirlendi ve oksidatif stresin indüklenmesi için bu doz kullanıldı. Farklı konsantrasyonlarda AP7Glu varlığında H2O2 uygulaması sadece H2O2 ile muamele edilmiş hücreler ile karşılaştırıldığında hücre canlılığını artırdı. H2O2 SOD ve katalaz enzim aktivitelerini kontrol grubuna göre azaltttı. H2O2’nin AP7Glu varlığında uygulanması ise sadece H2O2 ile muamele edilmiş hücreler ile karşılaştırıldığında bu enzimlerin aktivitelerinde artış gözlendi. Ayrıca, H2O2 antioksidan savunma sistemi ile ilişkili genlerin ekspresyon seviyelerini azaltırken Kaspaz genlerinin ekspresyon seviyelerini artırdı. Sadece H2O2 ile muamele edilmiş hücreler ile karşılaştırıldığında ise AP7Glu varlığında H2O2 uygulaması antioksidan savunma sistemi ile ilişkili genlerin ekspresyon seviyelerini arttırdı ve CASP8 ekspresyonunu azalttı. Sonuç: AP7Glu H2O2 ile indüklenmiş oksidatif stres ve nöronal hücre ölümüne karşı nöroprotektif bir etkiye sahiptir.

Anahtar Kelimeler:

Hidrojen peroksit, Oksidatif stres, Apigenin-7-Glikozit, SH-SY5Y

Apigenin-7-Glucoside Attenuates Hydrogen Peroxide-Induced Oxidative Stress and Neuronal Death in SH-SY5Y Cells Via Activation of Antioxidant Enzymes System and Inhibition of Caspases Genes Expression

Aims: In this study, it was aimed to investigate the possible neuroprotective effect of Apigenin-7-Glycoside (AP7Glu), a natural product with known anticancer and anti-inflammatory activity, against hydrogen proxide (H2O2)-induced oxidative stress in SH-SY5Y cells. Methods: The effect of H2O2 and AP7Glu on cell viability was determined by XTT analysis. SOD and catalase analyzes were performed to determine antioxidant enzyme activities. Expression levels of antioxidant defense system related genes and caspase genes were evaluated by qRT-PCR analysis. Results: After treatment of 300 µM H2O2 to SH-SY5Y cells for 24 hours, cell viability was determined as 63.18% and this dose was used to induce oxidative stress. H2O2 treatment in the presence of different concentrations of AP7Glu increased cell viability compared to H2O2-treated cells. And, H2O2 decreased SOD and catalase enzyme activities compared to control group. With the treatment of H2O2 in the presence of AP7Glu, an increase was observed in these enzymes activities compared to H2O2-treated cells. In addition, H2O2 decreased the expression levels of genes associated with antioxidant defense system and increased expression levels of Caspase genes. And, H2O2 treatment in the presence of AP7Glu increased the expression levels of genes associated with antioxidant defense system and decreased CASP8 expression compared to H2O2-treated cells. Conclusion: AP7Glu has a neuroprotective effect against H2O2-induced oxidative stress and neuronal cell death.

Keywords:

Apigenin-7-Glycoside, Hydrogen peroxide, Oxidative stress SH-SY5Y, Apigenin-7-Glikozit, Hidrojen peroksit, Oksidatif stres,

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