In-vitro cytotoxicity of white vinegar: Effects of different concentrations

In-vitro cytotoxicity of white vinegar: Effects of different concentrations

Aim: We compared the cytotoxic effects of different concentration of white vinegar, that easy to apply, unexpensive and effective on microorganisms. Method: The cells L929 mouse skin fibroblasts were used for the experiments. Cytotoxicity was evaluated colourimetrically by MTT [4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium bromide; thiazolyl blue] proliferation assay. Results: White vinegar was found to be moderately cytotoxic at its all concentrations, in the 10-minutes incubation period. While the other concentrations were moderately cytotoxic, only 100% white vinegar concentration was found to be severely cytotoxic, in the 24-hours incubation period. Conclusion: It was observed that white vinegar was moderately cytotoxic on L929 fibroblast at all concentrations, in the 10-minutes incubation period

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  • Basson, N., A. Quick & C. Thomas. (1992). Household products as sanitising agents in denture cleansing. The Journal of the Dental Association of South Africa= Die Tydskrif van die Tandheelkundige Vereniging van Suid-afrika, 47(10), 437-439.
  • Berridge, M.V., P.M. Herst & A.S. Tan. (2005). Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review, 11, 127-152.
  • Coelho, C., Y. Sousa & A. Dare. (2004). Denture‐related oral mucosal lesions in a Brazilian school of dentistry. Journal of oral rehabilitation, 31(2), 135-139.
  • Davenport, J.C. (1972). The denture surface. British Dental Journal, 133(3), 101-105.
  • Da Silva, F. C., Kimpara, E. T., Mancini, M. N. G., Balducci, I., Jorge, A. O. C., & Koga‐Ito, C. Y. (2008). Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. Journal of Prosthodontics: Implant, Esthetic and Reconstructive Dentistry, 17(8), 627-633.
  • DeWald, J.P. (1997). The use of extracted teeth for in vitro bonding studies: a review of infection control considerations. Dental Materials, 13(2), 74-81.
  • Dominici, J. T., Eleazer, P. D., Clark, S. J., Staat, R. H., & Scheetz, J. P. (2001). Disinfection/sterilization of extracted teeth for dental student use. Journal of Dental Education, 65(11), 1278-1281.
  • Estrela, C., Holland, R., Bernabé, P. F. E., Souza, V. D., & Estrela, C. R. (2004). Antimicrobial potential of medicaments used in healing process in dogs' teeth with apical periodontitis. Brazilian Dental Journal, 15(3), 181-185.
  • Jagger, D. & A. Harrison. (1995). Denture cleansing--the best approach. British Dental Journal, 178(11), 413-417.
  • Kulak, Y., A. Arikan & E. Kazazoglu. (1997). Existence of Candida albicans and microorganisms in denture stomatitis patients. Journal of Oral Rehabilitation, 24(10), 788-790.
  • Le Bars, P., Kouadio, A. A., N’goran, J. K., Badran, Z., & Soueidan, A. (2015). Relationship between removable prosthesis and some systemics disorders. The Journal of the Indian Prosthodontic Society, 15(4), 292.
  • Lyon, J. P., da Costa, S. C., Totti, V. M. G., Munhoz, M. F. V., & de Resende, M. A. (2006). Predisposing conditions for Candida spp. carriage in the oral cavity of denture wearers and individuals with natural teeth. Canadian Journal of Microbiology, 52(5), 462-467.
  • Lefebvre, C.A., K.L. Knoernschild & G.S. Schuster. (1994). Cytotoxicity of eluates from light-polymerized denture base resins. The Journal of Prosthetic Dentistry, 72(6), 644-650.
  • Lefebvre, C.A. & G.S. Schuster. (1994). Biocompatibility of visible light-cured resin systems in prosthodontics. Journal of Prosthetic Dentistry, 71(2), 178-185.
  • Meriç, G., J.E. Dahl & I.E. Ruyter. (2008). Cytotoxicity of silica–glass fiber reinforced composites. Dental Materials, 24(9), 1201-1206.
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
  • Nakamoto, K., M. Tamamoto & T. Hamada. (1991). Evaluation of denture cleansers with and without enzymes against Candida albicans. The Journal of Prosthetic Dentistry, 66(6), 792-795.
  • Nascimento, M. S., Silva, N., Catanozi, M. P. L. M., & Silva, K. C. (2003). Effects of different disinfection treatments on the natural microbiota of lettuce. Journal of Food Protection, 66(9), 1697-1700.
  • Palenik, C.J. & C.H. Miller. (1984). In vitro testing of three denture-cleaning systems. The Journal of Prosthetic Dentistry, 51(6), 751-754.
  • Preshaw, P. M., Walls, A. W. G., Jakubovics, N. S., Moynihan, P. J., Jepson, N. J. A., & Loewy, Z. (2011). Association of removable partial denture use with oral and systemic health. Journal of Dentistry, 39(11), 711-719.
  • Pinto, T. M. S., Neves, A. C. C., Leão, M. V. P., & Jorge, A. O. C. (2008). Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers. Journal of Applied Oral Science, 16, 385-390.
  • Ribeiro, D. G., Pavarina, A. C., Dovigo, L. N., Machado, A. L., Giampaolo, E. T., & Vergani, C. E. (2012). Prevalence of Candida spp. associated with bacteria species on complete dentures. Gerodontology, 29(3), 203-208.
  • Riss, T. L., Moravec, R. A., Niles, A. L., Duellman, S., Benink, H. A., Worzella, T. J., & Minor, L. (2016). Cell viability assays. Assay Guidance Manual.
  • Salles, A. E. S., Macedo, L. D., Fernandes, R. A., Silva‐Lovato, C. H., & Paranhos, H. D. F. (2007). Comparative analysis of biofilm levels in complete upper and lower dentures after brushing associated with specific denture paste and neutral soap. Gerodontology, 24(4), 217-223.
  • Schou, L., C. Wight, and C. Cumming. (1987). Oral hygiene habits, denture plaque, presence of yeasts and stomatitis in institutionalized elderly in Lathian, Scotland. Community dentistry and oral epidemiology, 15(2), 85-89.
  • Suresan, V., Mantri, S., Deogade, S., Sumathi, K., Panday, P., Galav, A., & Mishra, K. (2016). Denture hygiene knowledge, attitudes, and practices toward patient education in denture care among dental practitioners of Jabalpur city, Madhya Pradesh, India. The Journal of the Indian Prosthodontic Society, 16(1), 30.
  • Tijare, M., Smitha, D., Kasetty, S., Kallianpur, S., Gupta, S., & Amith, H. V. (2014). Vinegar as a disinfectant of extracted human teeth for dental educational use. Journal of Oral and Maxillofacial Pathology, 18(1), 14.
  • Ünlü, A., O.T. Altay & S. Sahmali. (1996). The role of denture cleansers on the whitening of acrylic resins. International Journal of Prosthodontics, 9(3).
  • Vijayakumar, C. & C.E. Wolf-Hall. (2002a). Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. Journal of Food Protection,. 65(10), 1646-1650.
  • Vijayakumar, C. & C. Wolf-Hall. (2002b). Minimum bacteriostatic and bactericidal concentrations of household sanitizers for Escherichia coli strains in tryptic soy broth. Food Microbiology, 19(4), 383-388.
  • Viana, P. S., Machado, A. L., Giampaolo, E. T., Pavarina, A. C., & Vergani, C. E. (2010). Disinfection of bovine enamel by microwave irradiation: effect on the surface microhardness and demineralization/remineralization processes. Caries Research, 44(4), 349-357.
  • Yang, Y., Zhang, H., Chai, Z., Chen, J., & Zhang, S. (2014). Multiple logistic regression analysis of risk factors associated with denture plaque and staining in chinese removable denture wearers over 40 years old in xi’an–a cross-sectional study. PLoS One, 9(2), e87749.
  • Yildirim-Bicer, A. Z., Peker, I., Akca, G., & Celik, I. (2014). In vitro antifungal evaluation of seven different disinfectants on acrylic resins. BioMed Research International