Bülent AKAY, Furkan SOYSAL, Zeynep YILMAZER HİTİT, Şule CAMCIOĞLU, Suna ERTUNÇ, Baran ÖZYURT

Karanlık fermentasyon ile patates besi ortamından biyohidrojen üretimi için uygun işletim koşullarının belirlenmesi ve kinetik analizi

Bu çalışmada çalkalama hızı, mikroorganizma aşı oranı, başlangıç substrat derişimi ve inkübasyon sıcaklığınınkaranlık fermentasyon ile biyohidrojen üretimine etkisi incelenmiştir. En uygun parametreler, çalkalama hızı 200devir/min, mikroorganizma aşı oranı 1:10 v/v, başlangıç substrat derişimi 32,4 g KOİ/L ve sıcaklık 37°C olarakbelirlenmiştir. Bu koşullarda 1257 mL H2 üretimi, 17,06 mL H2/ g KOİ verimi ve 6,441 mL H2/L.min maksimumH2 üretim hızı bulgularına ulaşılmıştır. Elemanter tepkime hız modeli için mertebe 1, hız sabitleri ise 28°C ve 37°Csıcaklıklar için sırasıyla 6,5x10-5 min-1 ve 7,92x10-5 min-1 olarak hesaplanmıştır. Arrhenius sabiti 9,74x1034 min-1ve aktivasyon enerjisi 229,20 kJ/mol olarak hesaplanmıştır. Michaelis-Menten modeli ile maksimum H2 üretimhızları 28°C ve 37°C sıcaklıklar için sırasıyla 11,47 mL H2/L.min ve 74,66 mL H2/L. min, bu sıcaklıklar içinMichaelis sabitleri sırasıyla 181,9 g KOİ/L ve 447,7 g KOİ/L, R2 değerleri ise 0,9458 ve 0,9505 olarakhesaplanmıştır. Modifiye Gompertz modeli ile R2 değerleri tüm çalışmalar için 0,99’dan büyük olarak elde edilmiş,en yüksek H2 üretimi potansiyelleri, 32,4 g KOİ/L başlangıç substrat derişiminde, 28°C ve 37°C sıcaklıklar içinsırasıyla 2,126 L H2/L ve 2,777 L H2/L olarak bulunmuş, en yüksek H2 üretim hızına 15,250 mL H2/L.min olarak37°C sıcaklık ve 27 g KOİ/L başlangıç substrat derişiminde ulaşılmıştır.

Determination of suitable operating conditions for biohydrogen production from potato production medium by dark fermentation and kinetic analysis

In this work, effects of agitation speed, inoculum ratio, initial substrate concentraion and incubation temperature were investigated for anaerobic biohydrogen production. The most suitable parameters were determined as agitation speed 200 rpm, microorganism inoculum ratio 1:10 v/v, initial substrate concentration 32.4 g COD/L and temperature 37°C. Under these conditions, 1257 mL H2 production, 17.06 mL H2/g COD yield and 6.441 mL H2/L maximum H2 production rate was reached. For first order elementary reaction rate model, reaction rate constants were calculated as 6.5x10-5 min-1 and 7.92x10-5 min-1 for temperatures of 28°C and 37°C, respectively. Arrhenius constant and activation energy were calculated as 9.74x1034 min-1 and 229.20 kJ/mol respectively. For MichaelisMenten model, maximum H2 production rates, Michaelis constants and R2 values were calculated as 11.47 mL H2/L.min, 74.66 mL H2/L.min, 181.9 g COD/L, 447.7 g COD/L and 0.9458, 0.9505 for 28ºC and 37°C temperatures, respectively. Using modified Gompertz model, all R2 values were obtained greater than 0.99, maximum H2 production potentials were found as 2.126 L H2/L, 2.777 L H2/L at 28°C and 37°C with 32.4 g COD/L, respectively. Maximum H2 production rate was reached as 15.250 mL H2/L.min at 37°C and 27 g COD/L initial substrate concentration.

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