Feyza TATLİ, Tuğba TABANLİGİL CALAM, Demet UZUN, Erdoğan HASDEMİR

[(1H-1,2,4-triazol-3-ylimin)metil]naftalen-2-ol modifiye platin elektrodu ile askorbik asit ve dopamin varlığında ürik asit tayini

Bu çalışmada, bir triazole türevi olan [(1H-1,2,4-triazol-3-ylimin)metil]naftalen-2-ol (TMN-2-ol) Schiff bazı ile platin (Pt) elektrot yüzeyi kaplanarak; TMN-2-ol/Pt elektrodu hazırlanmıştır. Kaplama işlemi, dönüşümlü voltametri (CV) yöntemi kullanılarak 1´10-3 M TMN-2-ol çözeltisi içerisinde, 100 mV s-1 tarama hızıyla -0,8 V ile +2,0 V arasında, 40 çevrim sayısı ile gerçekleştirilmiştir. Hazırlanan modifiye yüzey, CV, temas açısı (CAM) ve yansıtmalı absorpsiyon infrared spektroskopisi (RAIRS) yöntemleri kullanılarak karakterize edilmiştir. Hazırlanan ve karakterize edilen elektrodun askorbik asit (AA) ve dopamin (DA) varlığında ürik aside (ÜA) elektrokatalitik duyarlılığı, kare dalga voltametrisi (SWV) yöntemiyle incelenmiştir. Destek elektrolit, pH gibi optimum çalışma şartları belirlenmiştir. En uygun çalışma ortamı 0,1 M pH 7,2 fosfat tamponu (PBS) olarak bulunmuştur. TMN-2-ol/Pt elektrot ile ÜA için çalışma aralığı 1,23´10-6–1,51´10-5 M ve gözlenebilme sınırı (LOD) 1,56´10-8 M olarak belirlenmiştir. ÜA pik akımı üzerine çeşitli anyon ve katyonların girişim etkileri incelenmiştir. Modifiye elektrotla, insan kan serumunda standart ekleme yöntemi kullanarak düşük bağıl standart sapma değerleri ile ÜA analizi gerçekleştirilmiştir.

Anahtar Kelimeler:

Modifikasyon, karakterizasyon, elektrot, ürik asit

The determination of uric acid in the presence of ascorbic acid and dopamine using [(1H1,2,4-triazole-3-ylimino)methyl]naphthalene-2-ol modified platinum electrode

In this study [(1H-1,2,4-triazole-3-ylimino) methyl]naphtalene-2-ol (TMN-2-ol) was deposited at the platin electrode to fabricate a new sensor and used for the determination of uric acid in the presence of ascorbic acid (AA) and dopamine (DA) Electrocatalytic activity against uric acid (UA) was determined using modified electrode. Comparing with the bare Pt and TMN-2-ol modified Pt (TMN-2-ol/Pt) electrode, the TMN-2-ol /Pt electrode has higher catalytic activities towards the oxidation of UA in the presence of AA and DA. Figure A shows that electrode modification provided 5.54-fold increase at the precision.

Keywords:

Electrode modification reflection adsorption infrared spectroscopy, square wave voltammetry, platinum electrode, uric acid,

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